Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18487 | 3' | -57.4 | NC_004681.1 | + | 62078 | 0.71 | 0.410189 |
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Target: 5'- -gGUCGAACaGGugcaugcccGCGAUgaCGCUGCCGCc -3' miRNA: 3'- agUAGCUUGaCC---------CGCUG--GCGACGGCG- -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 62399 | 0.66 | 0.724002 |
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Target: 5'- aCAUcCGGguacuggcaugGCUGGGCGccgacACCGCccucuccGCCGCc -3' miRNA: 3'- aGUA-GCU-----------UGACCCGC-----UGGCGa------CGGCG- -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 65334 | 0.71 | 0.410189 |
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Target: 5'- uUCA-CGAGgUGcGGCaGGCCGCUGUCGg -3' miRNA: 3'- -AGUaGCUUgAC-CCG-CUGGCGACGGCg -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 66861 | 0.66 | 0.68257 |
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Target: 5'- gCGUCGAugUaGGCGG-CGUUGCCa- -3' miRNA: 3'- aGUAGCUugAcCCGCUgGCGACGGcg -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 66922 | 0.74 | 0.276624 |
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Target: 5'- gUCGUCGuugacuCUGuGGCGGCCcuGgUGCCGCg -3' miRNA: 3'- -AGUAGCuu----GAC-CCGCUGG--CgACGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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