Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18491 | 3' | -54.4 | NC_004681.1 | + | 64903 | 0.66 | 0.832505 |
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Target: 5'- cCUCggaggCGGgauGCAGCUCGUGgGGCgAGa -3' miRNA: 3'- -GAGaa---GCU---CGUCGAGUACgUCGgUCc -5' |
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| 18491 | 3' | -54.4 | NC_004681.1 | + | 31388 | 0.66 | 0.832505 |
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Target: 5'- -cCUUCGGGC--UUC-UGCGGCCAGa -3' miRNA: 3'- gaGAAGCUCGucGAGuACGUCGGUCc -5' |
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| 18491 | 3' | -54.4 | NC_004681.1 | + | 46301 | 0.66 | 0.81432 |
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Target: 5'- -aCggCGGGCAGCcauccugcaUCGUG-GGCCAGGc -3' miRNA: 3'- gaGaaGCUCGUCG---------AGUACgUCGGUCC- -5' |
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| 18491 | 3' | -54.4 | NC_004681.1 | + | 701 | 0.66 | 0.81432 |
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Target: 5'- ---aUCGAGgGGC-CAUGCGaggucugccuGCCGGGa -3' miRNA: 3'- gagaAGCUCgUCGaGUACGU----------CGGUCC- -5' |
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| 18491 | 3' | -54.4 | NC_004681.1 | + | 55966 | 0.67 | 0.755571 |
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Target: 5'- cCUCgUCGGGguGCcgGUGCucggcuGCCAGGc -3' miRNA: 3'- -GAGaAGCUCguCGagUACGu-----CGGUCC- -5' |
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| 18491 | 3' | -54.4 | NC_004681.1 | + | 60679 | 0.68 | 0.734876 |
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Target: 5'- cCUCgUCGAGCAccacGUUCGU-CGGCCAGcGg -3' miRNA: 3'- -GAGaAGCUCGU----CGAGUAcGUCGGUC-C- -5' |
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| 18491 | 3' | -54.4 | NC_004681.1 | + | 4425 | 0.7 | 0.572668 |
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Target: 5'- ---aUCGAGCAcaaaucGCgUCGUGCAGCgAGGu -3' miRNA: 3'- gagaAGCUCGU------CG-AGUACGUCGgUCC- -5' |
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| 18491 | 3' | -54.4 | NC_004681.1 | + | 59081 | 0.71 | 0.551187 |
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Target: 5'- gUCUUCGAGCAcCUgG-GCGGCCuGGu -3' miRNA: 3'- gAGAAGCUCGUcGAgUaCGUCGGuCC- -5' |
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| 18491 | 3' | -54.4 | NC_004681.1 | + | 52592 | 1.1 | 0.001388 |
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Target: 5'- cCUCUUCGAGCAGCUCAUGCAGCCAGGc -3' miRNA: 3'- -GAGAAGCUCGUCGAGUACGUCGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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