Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18492 | 3' | -50.1 | NC_004681.1 | + | 33354 | 0.7 | 0.86804 |
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Target: 5'- gGAAcugCGAgGACCCGCAUCACcuGCGc -3' miRNA: 3'- -CUUaa-GCU-CUGGGCGUAGUGcuUGCc -5' |
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| 18492 | 3' | -50.1 | NC_004681.1 | + | 16081 | 0.71 | 0.851551 |
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Target: 5'- -----gGAGGCCaGCAUCugGAccGCGGg -3' miRNA: 3'- cuuaagCUCUGGgCGUAGugCU--UGCC- -5' |
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| 18492 | 3' | -50.1 | NC_004681.1 | + | 39164 | 0.72 | 0.777125 |
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Target: 5'- ----cCGAGACCgGCAUC-CGGgccGCGGa -3' miRNA: 3'- cuuaaGCUCUGGgCGUAGuGCU---UGCC- -5' |
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| 18492 | 3' | -50.1 | NC_004681.1 | + | 49297 | 0.73 | 0.714714 |
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Target: 5'- ----cCGAGGCgCGCAagGCGGGCGGu -3' miRNA: 3'- cuuaaGCUCUGgGCGUagUGCUUGCC- -5' |
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| 18492 | 3' | -50.1 | NC_004681.1 | + | 55223 | 0.75 | 0.627328 |
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Target: 5'- cGAAUUCGAGACCCG---CugGGACGc -3' miRNA: 3'- -CUUAAGCUCUGGGCguaGugCUUGCc -5' |
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| 18492 | 3' | -50.1 | NC_004681.1 | + | 12457 | 0.75 | 0.616316 |
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Target: 5'- ---gUCGAGGCCC-CGgcCACGAGCGGu -3' miRNA: 3'- cuuaAGCUCUGGGcGUa-GUGCUUGCC- -5' |
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| 18492 | 3' | -50.1 | NC_004681.1 | + | 26173 | 0.75 | 0.605318 |
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Target: 5'- cGAAUUCGGuuCCCGgGUCACGAACc- -3' miRNA: 3'- -CUUAAGCUcuGGGCgUAGUGCUUGcc -5' |
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| 18492 | 3' | -50.1 | NC_004681.1 | + | 60786 | 0.77 | 0.508372 |
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Target: 5'- ---aUCGAGACggccguagaCCGCAccaUCACGAGCGGc -3' miRNA: 3'- cuuaAGCUCUG---------GGCGU---AGUGCUUGCC- -5' |
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| 18492 | 3' | -50.1 | NC_004681.1 | + | 53426 | 1.11 | 0.00423 |
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Target: 5'- cGAAUUCGAGACCCGCAUCACGAACGGu -3' miRNA: 3'- -CUUAAGCUCUGGGCGUAGUGCUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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