Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18493 | 5' | -51.8 | NC_004681.1 | + | 46003 | 0.66 | 0.931503 |
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Target: 5'- uGAU-CGGUGGGCGG--UCCUCgugcauuACCCc -3' miRNA: 3'- gCUGuGUUACCCGUCuaAGGAG-------UGGG- -5' |
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| 18493 | 5' | -51.8 | NC_004681.1 | + | 49303 | 0.66 | 0.920579 |
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Target: 5'- -cGCGCAAggcGGGCGGucugcacaaCCUCACCg -3' miRNA: 3'- gcUGUGUUa--CCCGUCuaa------GGAGUGGg -5' |
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| 18493 | 5' | -51.8 | NC_004681.1 | + | 50563 | 0.72 | 0.66618 |
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Target: 5'- gGACACGGUcGGGguGgg-CgUCGCCCa -3' miRNA: 3'- gCUGUGUUA-CCCguCuaaGgAGUGGG- -5' |
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| 18493 | 5' | -51.8 | NC_004681.1 | + | 50915 | 0.73 | 0.588282 |
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Target: 5'- gGugGCGGUGGGCGGGgucacagUUCCUgUugCCa -3' miRNA: 3'- gCugUGUUACCCGUCU-------AAGGA-GugGG- -5' |
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| 18493 | 5' | -51.8 | NC_004681.1 | + | 53787 | 1.13 | 0.001777 |
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Target: 5'- uCGACACAAUGGGCAGAUUCCUCACCCa -3' miRNA: 3'- -GCUGUGUUACCCGUCUAAGGAGUGGG- -5' |
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| 18493 | 5' | -51.8 | NC_004681.1 | + | 54357 | 0.68 | 0.847213 |
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Target: 5'- -uGCGCaAGUGGGCAcg--CCUCGCCa -3' miRNA: 3'- gcUGUG-UUACCCGUcuaaGGAGUGGg -5' |
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| 18493 | 5' | -51.8 | NC_004681.1 | + | 62209 | 0.72 | 0.644242 |
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Target: 5'- gGACAUggUGGGCuGGUaCCgcCACCUg -3' miRNA: 3'- gCUGUGuuACCCGuCUAaGGa-GUGGG- -5' |
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| 18493 | 5' | -51.8 | NC_004681.1 | + | 63436 | 0.72 | 0.66618 |
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Target: 5'- -cACGCGAauuUGGGCGGAUUUUUCAUCg -3' miRNA: 3'- gcUGUGUU---ACCCGUCUAAGGAGUGGg -5' |
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| 18493 | 5' | -51.8 | NC_004681.1 | + | 64776 | 0.68 | 0.855676 |
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Target: 5'- ---gGCGGUGGGCAuuccGGUcgUCUCGCCCc -3' miRNA: 3'- gcugUGUUACCCGU----CUAa-GGAGUGGG- -5' |
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| 18493 | 5' | -51.8 | NC_004681.1 | + | 70381 | 0.7 | 0.792027 |
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Target: 5'- aGACACGGaGGGCAuGAcagUCCUCcguGCCa -3' miRNA: 3'- gCUGUGUUaCCCGU-CUa--AGGAG---UGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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