Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18498 | 5' | -58.5 | NC_004681.1 | + | 68663 | 0.66 | 0.606648 |
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Target: 5'- gGCCcUGGAaggcaagGACG-AGCUGUCCuucGUGGAg -3' miRNA: 3'- -UGGcACCU-------CUGCgUCGACAGG---CACCU- -5' |
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| 18498 | 5' | -58.5 | NC_004681.1 | + | 28615 | 0.67 | 0.565865 |
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Target: 5'- uGCCGUGGAGAugacCGCGGggGUgcCCG-GGGu -3' miRNA: 3'- -UGGCACCUCU----GCGUCgaCA--GGCaCCU- -5' |
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| 18498 | 5' | -58.5 | NC_004681.1 | + | 60376 | 0.67 | 0.555512 |
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Target: 5'- cUCGUGGgcacGGugGCGGCgaaUGUCC-UGGAg -3' miRNA: 3'- uGGCACC----UCugCGUCG---ACAGGcACCU- -5' |
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| 18498 | 5' | -58.5 | NC_004681.1 | + | 662 | 0.68 | 0.534979 |
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Target: 5'- cGCC-UGGGGAUGCGGCcGUCacUGGAg -3' miRNA: 3'- -UGGcACCUCUGCGUCGaCAGgcACCU- -5' |
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| 18498 | 5' | -58.5 | NC_004681.1 | + | 16583 | 0.69 | 0.437248 |
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Target: 5'- uGCCG---GGGCGCGGCgccgaguaGUCCGUGGGg -3' miRNA: 3'- -UGGCaccUCUGCGUCGa-------CAGGCACCU- -5' |
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| 18498 | 5' | -58.5 | NC_004681.1 | + | 17073 | 0.71 | 0.366912 |
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Target: 5'- cUCGUuGAGGCGCAGCUcaGcCUGUGGAa -3' miRNA: 3'- uGGCAcCUCUGCGUCGA--CaGGCACCU- -5' |
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| 18498 | 5' | -58.5 | NC_004681.1 | + | 46381 | 0.71 | 0.32708 |
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Target: 5'- cGCCGUGGAcguaGACGCAGUgGUCCGc--- -3' miRNA: 3'- -UGGCACCU----CUGCGUCGaCAGGCaccu -5' |
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| 18498 | 5' | -58.5 | NC_004681.1 | + | 73615 | 0.78 | 0.118312 |
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Target: 5'- aGCgCGUGGAGACGCugcaaGGaCUGUCCGUGcGAc -3' miRNA: 3'- -UG-GCACCUCUGCG-----UC-GACAGGCAC-CU- -5' |
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| 18498 | 5' | -58.5 | NC_004681.1 | + | 56044 | 1.08 | 0.000911 |
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Target: 5'- cACCGUGGAGACGCAGCUGUCCGUGGAc -3' miRNA: 3'- -UGGCACCUCUGCGUCGACAGGCACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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