Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18499 | 3' | -57.1 | NC_004681.1 | + | 3083 | 0.69 | 0.530709 |
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Target: 5'- cGGGuACCGGCUGCCUcGAuGGCCGCg- -3' miRNA: 3'- -CUC-UGGCCGAUGGAcCUcUUGGUGgc -5' |
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| 18499 | 3' | -57.1 | NC_004681.1 | + | 51854 | 0.69 | 0.501266 |
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Target: 5'- cAGACCGGCguuacucuugcccgcGCCgGGGcGGCCGCCGa -3' miRNA: 3'- cUCUGGCCGa--------------UGGaCCUcUUGGUGGC- -5' |
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| 18499 | 3' | -57.1 | NC_004681.1 | + | 39165 | 0.7 | 0.4804 |
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Target: 5'- cGAGACCGGCauccggGCCgcGGAGAugCucGCCc -3' miRNA: 3'- -CUCUGGCCGa-----UGGa-CCUCUugG--UGGc -5' |
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| 18499 | 3' | -57.1 | NC_004681.1 | + | 5071 | 0.71 | 0.423293 |
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Target: 5'- uGGAauUCGGCgaugugcGCgUGGAGAACCACCa -3' miRNA: 3'- cUCU--GGCCGa------UGgACCUCUUGGUGGc -5' |
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| 18499 | 3' | -57.1 | NC_004681.1 | + | 37177 | 0.71 | 0.405178 |
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Target: 5'- uGGAUCGGCUgguucugagGCCgcugGGAuGAGCCACCc -3' miRNA: 3'- cUCUGGCCGA---------UGGa---CCU-CUUGGUGGc -5' |
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| 18499 | 3' | -57.1 | NC_004681.1 | + | 38818 | 0.71 | 0.399839 |
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Target: 5'- cGAGACCauccgggccuacggcGGCaGCgUGGAGGGCUACCu -3' miRNA: 3'- -CUCUGG---------------CCGaUGgACCUCUUGGUGGc -5' |
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| 18499 | 3' | -57.1 | NC_004681.1 | + | 1867 | 0.76 | 0.217259 |
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Target: 5'- uGAGGagGGCgaugGCCUGGGGAACUACCu -3' miRNA: 3'- -CUCUggCCGa---UGGACCUCUUGGUGGc -5' |
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| 18499 | 3' | -57.1 | NC_004681.1 | + | 12420 | 0.76 | 0.211775 |
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Target: 5'- cGAGACCGGCU-CCUGGAGcACgaugguCACCu -3' miRNA: 3'- -CUCUGGCCGAuGGACCUCuUG------GUGGc -5' |
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| 18499 | 3' | -57.1 | NC_004681.1 | + | 56125 | 1.08 | 0.001197 |
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Target: 5'- cGAGACCGGCUACCUGGAGAACCACCGc -3' miRNA: 3'- -CUCUGGCCGAUGGACCUCUUGGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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