miRNA: 3'- -GUGUCUGUUGaaGAagGCGUCGCGuG- -5'
miRNA: 3'- -GUGUCUGU-------------UGA-----AGAAGgCG---UCGCGUG- -5'
miRNA: 3'- -GUGUCUGUUGAAGAAGGCGUCGCGUG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18500 | 3' | -52.1 | NC_004681.1 | + | 75246 | 0.67 | 0.854816 |
|
Target: 5'- cCACGGGCAACa-CUuaCGUAGCGCuCa -3' miRNA: 3'- -GUGUCUGUUGaaGAagGCGUCGCGuG- -5' |
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| 18500 | 3' | -52.1 | NC_004681.1 | + | 36121 | 0.66 | 0.886029 |
|
Target: 5'- gCGCGGGCAaucuugcgccgccACUucucgUCUUCgGUuucGGCGCACa -3' miRNA: 3'- -GUGUCUGU-------------UGA-----AGAAGgCG---UCGCGUG- -5' |
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| 18500 | 3' | -52.1 | NC_004681.1 | + | 56397 | 1.11 | 0.001927 |
|
Target: 5'- aCACAGACAACUUCUUCCGCAGCGCACa -3' miRNA: 3'- -GUGUCUGUUGAAGAAGGCGUCGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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