Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18501 | 3' | -64.5 | NC_004681.1 | + | 40570 | 0.68 | 0.2606 |
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Target: 5'- gGCGGCGUcgGCCGCGGcguucaaugucaucGGCcgccucaagggcgccGAGGUCCAa -3' miRNA: 3'- aUGCUGCA--CGGCGCC--------------CCG---------------CUCCGGGU- -5' |
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| 18501 | 3' | -64.5 | NC_004681.1 | + | 38226 | 0.67 | 0.306133 |
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Target: 5'- --aGACGUcCCGCGGcGGCGccauAGGCaCCGu -3' miRNA: 3'- augCUGCAcGGCGCC-CCGC----UCCG-GGU- -5' |
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| 18501 | 3' | -64.5 | NC_004681.1 | + | 69222 | 0.67 | 0.306133 |
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Target: 5'- gGCGAgGgagGCCaggGCGcGGGCGAGGCg-- -3' miRNA: 3'- aUGCUgCa--CGG---CGC-CCCGCUCCGggu -5' |
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| 18501 | 3' | -64.5 | NC_004681.1 | + | 32780 | 0.67 | 0.306133 |
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Target: 5'- cGCGACGU-CgGCGGGGUacccgGAccacaugucGGCCCAg -3' miRNA: 3'- aUGCUGCAcGgCGCCCCG-----CU---------CCGGGU- -5' |
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| 18501 | 3' | -64.5 | NC_004681.1 | + | 24839 | 0.66 | 0.320422 |
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Target: 5'- -uCGGCGUcgGCgGCGcGGGCGGcGGCCg- -3' miRNA: 3'- auGCUGCA--CGgCGC-CCCGCU-CCGGgu -5' |
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| 18501 | 3' | -64.5 | NC_004681.1 | + | 12489 | 0.66 | 0.327753 |
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Target: 5'- gACGAgCGUcGCCGCGGuGGUGGuGCCa- -3' miRNA: 3'- aUGCU-GCA-CGGCGCC-CCGCUcCGGgu -5' |
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| 18501 | 3' | -64.5 | NC_004681.1 | + | 67355 | 0.66 | 0.335206 |
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Target: 5'- -uCGGCGuUGCgGUGcGGCGGGGCCa- -3' miRNA: 3'- auGCUGC-ACGgCGCcCCGCUCCGGgu -5' |
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| 18501 | 3' | -64.5 | NC_004681.1 | + | 18392 | 0.66 | 0.342783 |
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Target: 5'- gGCGGCG-GCCGCGGcGGCaaacGCCUu -3' miRNA: 3'- aUGCUGCaCGGCGCC-CCGcuc-CGGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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