Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18502 | 3' | -52.9 | NC_004681.1 | + | 24460 | 0.66 | 0.895479 |
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Target: 5'- cUGUCCGGCAAcGAGGcgCCG-GGA-GAGu -3' miRNA: 3'- -ACAGGCUGUU-CUUCa-GGCaCCUgCUU- -5' |
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| 18502 | 3' | -52.9 | NC_004681.1 | + | 46351 | 0.66 | 0.895479 |
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Target: 5'- aGUCauuGACGGGggGUUgGUaGACGAAg -3' miRNA: 3'- aCAGg--CUGUUCuuCAGgCAcCUGCUU- -5' |
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| 18502 | 3' | -52.9 | NC_004681.1 | + | 4038 | 0.67 | 0.83997 |
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Target: 5'- -cUCCGAUgGAGGAGgacCCGUGGaACGAGg -3' miRNA: 3'- acAGGCUG-UUCUUCa--GGCACC-UGCUU- -5' |
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| 18502 | 3' | -52.9 | NC_004681.1 | + | 36787 | 0.67 | 0.831106 |
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Target: 5'- cGUCCccGAUGAGGAGcCCGUGG-UGAu -3' miRNA: 3'- aCAGG--CUGUUCUUCaGGCACCuGCUu -5' |
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| 18502 | 3' | -52.9 | NC_004681.1 | + | 56045 | 0.68 | 0.773897 |
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Target: 5'- --aCCGugGAGAcgcagcuGUCCGUGGACu-- -3' miRNA: 3'- acaGGCugUUCUu------CAGGCACCUGcuu -5' |
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| 18502 | 3' | -52.9 | NC_004681.1 | + | 22707 | 0.71 | 0.65717 |
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Target: 5'- gUGUCCGugGAGAAGgagGUGGGCa-- -3' miRNA: 3'- -ACAGGCugUUCUUCaggCACCUGcuu -5' |
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| 18502 | 3' | -52.9 | NC_004681.1 | + | 1277 | 0.72 | 0.601203 |
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Target: 5'- aUGUCgGAgggcgccCAGGAAGUCUG-GGACGAGc -3' miRNA: 3'- -ACAGgCU-------GUUCUUCAGGCaCCUGCUU- -5' |
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| 18502 | 3' | -52.9 | NC_004681.1 | + | 53266 | 0.76 | 0.38 |
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Target: 5'- aUGUCgCGGCcaugAGGAAGUCCGUGGcCGGGu -3' miRNA: 3'- -ACAG-GCUG----UUCUUCAGGCACCuGCUU- -5' |
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| 18502 | 3' | -52.9 | NC_004681.1 | + | 57055 | 1.07 | 0.003564 |
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Target: 5'- uUGUCCGACAAGAAGUCCGUGGACGAAg -3' miRNA: 3'- -ACAGGCUGUUCUUCAGGCACCUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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