Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18503 | 5' | -63.2 | NC_004681.1 | + | 61569 | 0.67 | 0.350523 |
Target: 5'- -cCUGGGGGGUcagaacGGAacgUCGUcauCGCCCCAc -3' miRNA: 3'- uuGAUCCCCCG------CCU---AGCG---GCGGGGUc -5' |
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18503 | 5' | -63.2 | NC_004681.1 | + | 75808 | 0.73 | 0.127312 |
Target: 5'- ----uGGGGGUGGuugcggucucgaaAUCGCCGCCUCGGg -3' miRNA: 3'- uugauCCCCCGCC-------------UAGCGGCGGGGUC- -5' |
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18503 | 5' | -63.2 | NC_004681.1 | + | 26328 | 0.79 | 0.045917 |
Target: 5'- cGCgUAGGGGGCGGGcaUCGCCGCCguggcgggCCAGg -3' miRNA: 3'- uUG-AUCCCCCGCCU--AGCGGCGG--------GGUC- -5' |
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18503 | 5' | -63.2 | NC_004681.1 | + | 57202 | 1.06 | 0.000398 |
Target: 5'- uAACUAGGGGGCGGAUCGCCGCCCCAGg -3' miRNA: 3'- -UUGAUCCCCCGCCUAGCGGCGGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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