Results 81 - 85 of 85 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18504 | 3' | -56.2 | NC_004681.1 | + | 69382 | 0.71 | 0.497795 |
Target: 5'- aGCUCGuccACGCGGUCCaUCaGaUCGGCCCa -3' miRNA: 3'- gCGAGC---UGCGUUAGG-AGcC-GGUUGGG- -5' |
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18504 | 3' | -56.2 | NC_004681.1 | + | 69740 | 0.69 | 0.590498 |
Target: 5'- -cUUUGGagaAAUUCUCGGCCGACCCg -3' miRNA: 3'- gcGAGCUgcgUUAGGAGCCGGUUGGG- -5' |
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18504 | 3' | -56.2 | NC_004681.1 | + | 71733 | 0.69 | 0.611636 |
Target: 5'- aCGCUCcgcGCGUAGUCCUCcgGGUCAAUUg -3' miRNA: 3'- -GCGAGc--UGCGUUAGGAG--CCGGUUGGg -5' |
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18504 | 3' | -56.2 | NC_004681.1 | + | 75392 | 0.66 | 0.782689 |
Target: 5'- gGCUaCGACaGCGAgcacgagaccgaauUCCgcgCGGCCGuacucgcggcgcaGCCCa -3' miRNA: 3'- gCGA-GCUG-CGUU--------------AGGa--GCCGGU-------------UGGG- -5' |
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18504 | 3' | -56.2 | NC_004681.1 | + | 75491 | 0.68 | 0.63284 |
Target: 5'- cCGCUCagcaGACGCGAgcucauacgCCagaGGCuCAACCCg -3' miRNA: 3'- -GCGAG----CUGCGUUa--------GGag-CCG-GUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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