miRNA: 3'- gCG-AGCUGCGUuAGGAGcCGGUUG-GG- -5'
miRNA: 3'- gCGAGCUGCgUUAGgagCCGGUugGG- -5'
miRNA: 3'- gCGAGCUGCGuuaGGAGCCGGU------UGGG- -5'
miRNA: 3'- gCGAGC---UGCGUUa----GGag-CCGGUUGGG- -5'
miRNA: 3'- gCGAGCUgCGU---------------UAGgAGCCGGUUGGG- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18504 | 3' | -56.2 | NC_004681.1 | + | 18949 | 0.7 | 0.517914 |
|
Target: 5'- uGCgUCGAUGUAuUCCUUcGCCAGCuCCg -3' miRNA: 3'- gCG-AGCUGCGUuAGGAGcCGGUUG-GG- -5' |
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| 18504 | 3' | -56.2 | NC_004681.1 | + | 41069 | 0.7 | 0.507812 |
|
Target: 5'- aGCUCGGCGaGGUCaa-GGCCAugCUg -3' miRNA: 3'- gCGAGCUGCgUUAGgagCCGGUugGG- -5' |
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| 18504 | 3' | -56.2 | NC_004681.1 | + | 44077 | 0.71 | 0.497795 |
|
Target: 5'- uGUUUGACGU---CCUCGGCCAugggguACCUa -3' miRNA: 3'- gCGAGCUGCGuuaGGAGCCGGU------UGGG- -5' |
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| 18504 | 3' | -56.2 | NC_004681.1 | + | 52969 | 0.71 | 0.497795 |
|
Target: 5'- gGCUCGggcACGCGGgcaggCCgauGGCCAGCCUu -3' miRNA: 3'- gCGAGC---UGCGUUa----GGag-CCGGUUGGG- -5' |
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| 18504 | 3' | -56.2 | NC_004681.1 | + | 1422 | 0.77 | 0.218668 |
|
Target: 5'- gGCUUGAgGCAgcccuugucggcgagGUCgUCGGCCAGCUCg -3' miRNA: 3'- gCGAGCUgCGU---------------UAGgAGCCGGUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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