Results 101 - 109 of 109 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18506 | 3' | -63.2 | NC_004681.1 | + | 22491 | 0.69 | 0.282891 |
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Target: 5'- gCGGCCGGgGU---GACCaGCGUCCACu -3' miRNA: 3'- -GCCGGCUgCGguuCUGGcCGCGGGUG- -5' |
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| 18506 | 3' | -63.2 | NC_004681.1 | + | 33454 | 0.69 | 0.282891 |
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Target: 5'- gCGGCCGGucucaGCCAgcgugaugugcGGGCgGGUGCCCu- -3' miRNA: 3'- -GCCGGCUg----CGGU-----------UCUGgCCGCGGGug -5' |
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| 18506 | 3' | -63.2 | NC_004681.1 | + | 42122 | 0.69 | 0.282891 |
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Target: 5'- aGGuuGGCGCgcaCGAGGuuGGCGUCgACa -3' miRNA: 3'- gCCggCUGCG---GUUCUggCCGCGGgUG- -5' |
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| 18506 | 3' | -63.2 | NC_004681.1 | + | 16496 | 0.69 | 0.272469 |
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Target: 5'- uGGCCuggucacccuuggagGugGCCGAGGCaacccacuUGGCGCCCuCg -3' miRNA: 3'- gCCGG---------------CugCGGUUCUG--------GCCGCGGGuG- -5' |
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| 18506 | 3' | -63.2 | NC_004681.1 | + | 6483 | 0.69 | 0.270549 |
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Target: 5'- gGGCCuccucccccucgacaGCGCCAucGGGCgUGGCGCCCAa -3' miRNA: 3'- gCCGGc--------------UGCGGU--UCUG-GCCGCGGGUg -5' |
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| 18506 | 3' | -63.2 | NC_004681.1 | + | 44483 | 0.69 | 0.269912 |
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Target: 5'- aGGgUGAguUGCCcGGACgGGCGCCCGu -3' miRNA: 3'- gCCgGCU--GCGGuUCUGgCCGCGGGUg -5' |
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| 18506 | 3' | -63.2 | NC_004681.1 | + | 12094 | 0.69 | 0.263604 |
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Target: 5'- uCGGCUugGugGCCuuguAGccAgCGGUGCCCACg -3' miRNA: 3'- -GCCGG--CugCGGu---UC--UgGCCGCGGGUG- -5' |
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| 18506 | 3' | -63.2 | NC_004681.1 | + | 17943 | 0.7 | 0.245399 |
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Target: 5'- -cGCCGggGCGCCcggguugguGAGGCCGGUGCCUugcGCg -3' miRNA: 3'- gcCGGC--UGCGG---------UUCUGGCCGCGGG---UG- -5' |
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| 18506 | 3' | -63.2 | NC_004681.1 | + | 994 | 0.75 | 0.106652 |
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Target: 5'- aGGCgGACGCCAGGG-CGGCuuCCCGCu -3' miRNA: 3'- gCCGgCUGCGGUUCUgGCCGc-GGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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