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miRNA: 3'- -ACCACGAGcu--GCUCCGG---------CGGC-----GACGC- -5'
miRNA: 3'- accaCGAG---CUGCUC-CGGCGGCGaCGC- -5'
miRNA: 3'- aCCACG--AGCUGCu-CCG--GCGGCGaCGC- -5'
miRNA: 3'- aCCACGag--CUGCU----CCGGCGgCGaCGC- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 18511 | 3' | -62.2 | NC_004681.1 | + | 17153 | 0.66 | 0.43322 |
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Target: 5'- uUGGUGCUCuuguCGAGGUCgaaguccuuGCCGaacuuCUGCGa -3' miRNA: 3'- -ACCACGAGcu--GCUCCGG---------CGGC-----GACGC- -5' |
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| 18511 | 3' | -62.2 | NC_004681.1 | + | 12497 | 0.66 | 0.424267 |
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Target: 5'- ---gGCUCaccGACGAGcGUCGCCGCgGUGg -3' miRNA: 3'- accaCGAG---CUGCUC-CGGCGGCGaCGC- -5' |
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| 18511 | 3' | -62.2 | NC_004681.1 | + | 33286 | 0.66 | 0.470089 |
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Target: 5'- gGGUGUcaUCGACGccGGUcgCGCCGCgGUGu -3' miRNA: 3'- aCCACG--AGCUGCu-CCG--GCGGCGaCGC- -5' |
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| 18511 | 3' | -62.2 | NC_004681.1 | + | 14671 | 0.66 | 0.470089 |
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Target: 5'- cGGUGCcgaaGGCGAcugcGGCCGUgGUgGCGg -3' miRNA: 3'- aCCACGag--CUGCU----CCGGCGgCGaCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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