Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18511 | 3' | -62.2 | NC_004681.1 | + | 61777 | 0.67 | 0.389595 |
Target: 5'- uUGGagacCUCGGCGGcGGCCuCCGCgGCGg -3' miRNA: 3'- -ACCac--GAGCUGCU-CCGGcGGCGaCGC- -5' |
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18511 | 3' | -62.2 | NC_004681.1 | + | 62070 | 0.7 | 0.25176 |
Target: 5'- aGGUGCaugcccgCGAUGAcGCUGCCGCccgGCGa -3' miRNA: 3'- aCCACGa------GCUGCUcCGGCGGCGa--CGC- -5' |
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18511 | 3' | -62.2 | NC_004681.1 | + | 67745 | 0.67 | 0.398088 |
Target: 5'- aUGGUGUugugcgacUCGAUGAGGCgCGUCGuCUuggacGCGa -3' miRNA: 3'- -ACCACG--------AGCUGCUCCG-GCGGC-GA-----CGC- -5' |
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18511 | 3' | -62.2 | NC_004681.1 | + | 74696 | 0.66 | 0.470089 |
Target: 5'- uUGGUGCUUGGCaauGGCUuaUGCgugGCGu -3' miRNA: 3'- -ACCACGAGCUGcu-CCGGcgGCGa--CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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