Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18519 | 3' | -58.3 | NC_004681.1 | + | 54946 | 0.66 | 0.621599 |
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Target: 5'- gGCGUCaGCcGGUCgCAUGAUgaGCUGGUc -3' miRNA: 3'- aUGUAG-CGuCCGGgGUACUG--CGACCA- -5' |
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| 18519 | 3' | -58.3 | NC_004681.1 | + | 33461 | 0.66 | 0.621599 |
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Target: 5'- -uCGUUGCGGGCCaguggCCGUGGCugaucCUGGUg -3' miRNA: 3'- auGUAGCGUCCGG-----GGUACUGc----GACCA- -5' |
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| 18519 | 3' | -58.3 | NC_004681.1 | + | 46317 | 0.66 | 0.578837 |
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Target: 5'- cUGCAUCGUGGGCCaggcaCUGUGgaaGCUGGg -3' miRNA: 3'- -AUGUAGCGUCCGG-----GGUACug-CGACCa -5' |
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| 18519 | 3' | -58.3 | NC_004681.1 | + | 55273 | 0.66 | 0.578837 |
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Target: 5'- -cCGUCGCGGGCgaUCCAUGGCuucGCcGGUu -3' miRNA: 3'- auGUAGCGUCCG--GGGUACUG---CGaCCA- -5' |
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| 18519 | 3' | -58.3 | NC_004681.1 | + | 25182 | 0.67 | 0.557676 |
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Target: 5'- gGCccCGguCAGGCCCCuggcGGCGCUGGc -3' miRNA: 3'- aUGuaGC--GUCCGGGGua--CUGCGACCa -5' |
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| 18519 | 3' | -58.3 | NC_004681.1 | + | 44148 | 0.67 | 0.505876 |
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Target: 5'- --uGUgGCAGGCCCCGcaGugGgaGGUg -3' miRNA: 3'- augUAgCGUCCGGGGUa-CugCgaCCA- -5' |
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| 18519 | 3' | -58.3 | NC_004681.1 | + | 40117 | 0.68 | 0.495755 |
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Target: 5'- gACGUCugcguauuccugGCAGGCCaaGcGGCGCUGGUu -3' miRNA: 3'- aUGUAG------------CGUCCGGggUaCUGCGACCA- -5' |
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| 18519 | 3' | -58.3 | NC_004681.1 | + | 58748 | 0.7 | 0.36559 |
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Target: 5'- cGCggCGCAGGCggCCGUGGCGCaGGa -3' miRNA: 3'- aUGuaGCGUCCGg-GGUACUGCGaCCa -5' |
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| 18519 | 3' | -58.3 | NC_004681.1 | + | 47491 | 0.71 | 0.309944 |
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Target: 5'- gACGUCGCAGGaCCgcgucgCCGUGGCGCguUGGc -3' miRNA: 3'- aUGUAGCGUCC-GG------GGUACUGCG--ACCa -5' |
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| 18519 | 3' | -58.3 | NC_004681.1 | + | 64695 | 1.06 | 0.000992 |
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Target: 5'- aUACAUCGCAGGCCCCAUGACGCUGGUg -3' miRNA: 3'- -AUGUAGCGUCCGGGGUACUGCGACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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