Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18520 | 3' | -49.9 | NC_004681.1 | + | 48051 | 0.66 | 0.97699 |
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Target: 5'- cUCuGGAUGCCUucuCUcauUGGAGuGUGAUGGa -3' miRNA: 3'- -GGuCCUACGGAu--GG---ACUUC-UACUACU- -5' |
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| 18520 | 3' | -49.9 | NC_004681.1 | + | 714 | 0.66 | 0.974236 |
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Target: 5'- gCgAGGucUGCCUGCCgggaaucGAGGAcgacUGGUGAc -3' miRNA: 3'- -GgUCCu-ACGGAUGGa------CUUCU----ACUACU- -5' |
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| 18520 | 3' | -49.9 | NC_004681.1 | + | 26819 | 0.66 | 0.968022 |
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Target: 5'- gCCGGGGcggGCUUACCgagGAacGGGUcaGAUGAg -3' miRNA: 3'- -GGUCCUa--CGGAUGGa--CU--UCUA--CUACU- -5' |
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| 18520 | 3' | -49.9 | NC_004681.1 | + | 25971 | 0.68 | 0.921084 |
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Target: 5'- uCCGGGGUGUCgaaCUGGucGAUGGUGu -3' miRNA: 3'- -GGUCCUACGGaugGACUu-CUACUACu -5' |
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| 18520 | 3' | -49.9 | NC_004681.1 | + | 48862 | 0.7 | 0.863388 |
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Target: 5'- uCCAGGuguaGCCgccguCCUGGAGGUGcUGGa -3' miRNA: 3'- -GGUCCua--CGGau---GGACUUCUACuACU- -5' |
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| 18520 | 3' | -49.9 | NC_004681.1 | + | 62606 | 0.71 | 0.828376 |
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Target: 5'- gCCAGGGUGCCcaugGCCUGGgccAUGAUc- -3' miRNA: 3'- -GGUCCUACGGa---UGGACUuc-UACUAcu -5' |
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| 18520 | 3' | -49.9 | NC_004681.1 | + | 37851 | 0.71 | 0.799844 |
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Target: 5'- -uGGGAUGCCauccAUCaGGAGGUGGUGAa -3' miRNA: 3'- ggUCCUACGGa---UGGaCUUCUACUACU- -5' |
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| 18520 | 3' | -49.9 | NC_004681.1 | + | 51960 | 0.72 | 0.748818 |
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Target: 5'- aCCAGG-UGCCUGCCgcGAGGAgucgGcUGAa -3' miRNA: 3'- -GGUCCuACGGAUGGa-CUUCUa---CuACU- -5' |
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| 18520 | 3' | -49.9 | NC_004681.1 | + | 65677 | 1.13 | 0.00295 |
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Target: 5'- aCCAGGAUGCCUACCUGAAGAUGAUGAa -3' miRNA: 3'- -GGUCCUACGGAUGGACUUCUACUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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