Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18523 | 3' | -53.7 | NC_004681.1 | + | 46791 | 0.66 | 0.84185 |
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Target: 5'- uCAUCccGCGcGCCGUCgAGGaauacucGGAGCGCu -3' miRNA: 3'- -GUAGcuUGUuCGGUAG-UCC-------CCUCGCG- -5' |
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| 18523 | 3' | -53.7 | NC_004681.1 | + | 49653 | 0.68 | 0.777453 |
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Target: 5'- -cUCGGGCcuGCCcgCGGGGGAaggccaGgGCg -3' miRNA: 3'- guAGCUUGuuCGGuaGUCCCCU------CgCG- -5' |
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| 18523 | 3' | -53.7 | NC_004681.1 | + | 56775 | 0.78 | 0.247983 |
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Target: 5'- --aCGAugAAGCCuUCGGGGauGAGCGCa -3' miRNA: 3'- guaGCUugUUCGGuAGUCCC--CUCGCG- -5' |
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| 18523 | 3' | -53.7 | NC_004681.1 | + | 59803 | 0.67 | 0.815864 |
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Target: 5'- uGUUG-GCAGGCCGcacucccagCAGGGG-GCGUg -3' miRNA: 3'- gUAGCuUGUUCGGUa--------GUCCCCuCGCG- -5' |
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| 18523 | 3' | -53.7 | NC_004681.1 | + | 64120 | 0.79 | 0.223598 |
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Target: 5'- ---gGGACugAGGCUGUCAGGGGAGCGUc -3' miRNA: 3'- guagCUUG--UUCGGUAGUCCCCUCGCG- -5' |
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| 18523 | 3' | -53.7 | NC_004681.1 | + | 67457 | 1.13 | 0.001173 |
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Target: 5'- cCAUCGAACAAGCCAUCAGGGGAGCGCg -3' miRNA: 3'- -GUAGCUUGUUCGGUAGUCCCCUCGCG- -5' |
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| 18523 | 3' | -53.7 | NC_004681.1 | + | 68784 | 0.66 | 0.867608 |
|
Target: 5'- gGUCGAccauagACAGGCCG-CAGaGGAGCu- -3' miRNA: 3'- gUAGCU------UGUUCGGUaGUCcCCUCGcg -5' |
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| 18523 | 3' | -53.7 | NC_004681.1 | + | 74158 | 0.69 | 0.694242 |
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Target: 5'- -cUCGAAgaguCGGGCCAaCAGGuGGccAGCGCa -3' miRNA: 3'- guAGCUU----GUUCGGUaGUCC-CC--UCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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