Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18523 | 5' | -53.9 | NC_004681.1 | + | 20639 | 0.67 | 0.826407 |
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Target: 5'- cGGCGGUUgcugcgUUGUACgGGUCUGCgcggagGCGUg -3' miRNA: 3'- cCUGCUAA------GACAUG-CCGGACGa-----CGCG- -5' |
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| 18523 | 5' | -53.9 | NC_004681.1 | + | 40377 | 0.67 | 0.817369 |
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Target: 5'- uGGCuucggCUGUACuGGCuaCUGCUGUGCg -3' miRNA: 3'- cCUGcuaa-GACAUG-CCG--GACGACGCG- -5' |
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| 18523 | 5' | -53.9 | NC_004681.1 | + | 49414 | 0.68 | 0.779438 |
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Target: 5'- cGGACGGUgaggUUGUGCagaccGCCcGCcuUGCGCg -3' miRNA: 3'- -CCUGCUAa---GACAUGc----CGGaCG--ACGCG- -5' |
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| 18523 | 5' | -53.9 | NC_004681.1 | + | 9946 | 0.68 | 0.779438 |
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Target: 5'- uGGGcCGGUUCgucuucgccuUGCGGCC-GCUGCGg -3' miRNA: 3'- -CCU-GCUAAGac--------AUGCCGGaCGACGCg -5' |
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| 18523 | 5' | -53.9 | NC_004681.1 | + | 1595 | 0.68 | 0.769561 |
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Target: 5'- gGGACGAgua--UugGGCCauggugGUUGCGCa -3' miRNA: 3'- -CCUGCUaagacAugCCGGa-----CGACGCG- -5' |
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| 18523 | 5' | -53.9 | NC_004681.1 | + | 49627 | 0.7 | 0.65426 |
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Target: 5'- aGGGCGAUgucggcacccUUGUcgaccAUGGCCUgGUUGCGCa -3' miRNA: 3'- -CCUGCUAa---------GACA-----UGCCGGA-CGACGCG- -5' |
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| 18523 | 5' | -53.9 | NC_004681.1 | + | 24005 | 0.71 | 0.568029 |
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Target: 5'- uGACGAUUgUGUcgagcAUGGCCUGgaUGCGUc -3' miRNA: 3'- cCUGCUAAgACA-----UGCCGGACg-ACGCG- -5' |
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| 18523 | 5' | -53.9 | NC_004681.1 | + | 4235 | 0.76 | 0.355641 |
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Target: 5'- cGAUGGggUUGUugcgggccucaGCGGCCUGCuUGCGCa -3' miRNA: 3'- cCUGCUaaGACA-----------UGCCGGACG-ACGCG- -5' |
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| 18523 | 5' | -53.9 | NC_004681.1 | + | 16299 | 0.66 | 0.883671 |
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Target: 5'- uGGCcGUUCUGcuUGGCCUGCUcUGCc -3' miRNA: 3'- cCUGcUAAGACauGCCGGACGAcGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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