Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18525 | 5' | -53.1 | NC_004681.1 | + | 2550 | 0.7 | 0.705956 |
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Target: 5'- gCCGAAGAguGGGCgGACgaUGUCGUcGCg -3' miRNA: 3'- -GGCUUCU--UCCGaCUGgaGCAGCAcUG- -5' |
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| 18525 | 5' | -53.1 | NC_004681.1 | + | 38042 | 0.71 | 0.651257 |
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Target: 5'- gCCGAGGGccauccggccucgGGGCUGA-CUCGcUCGcUGACg -3' miRNA: 3'- -GGCUUCU-------------UCCGACUgGAGC-AGC-ACUG- -5' |
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| 18525 | 5' | -53.1 | NC_004681.1 | + | 52851 | 0.71 | 0.64152 |
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Target: 5'- uCCGGgcGGAAGGCUGGCCaUCGgccugcccgCGUGcCc -3' miRNA: 3'- -GGCU--UCUUCCGACUGG-AGCa--------GCACuG- -5' |
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| 18525 | 5' | -53.1 | NC_004681.1 | + | 5774 | 0.73 | 0.533302 |
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Target: 5'- uUGAAGAAgcggaucGGCacGACCUCGUCGUcGGCg -3' miRNA: 3'- gGCUUCUU-------CCGa-CUGGAGCAGCA-CUG- -5' |
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| 18525 | 5' | -53.1 | NC_004681.1 | + | 21309 | 0.83 | 0.140533 |
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Target: 5'- gCUGAAGAAGGC-GACCaUCGUCGUGgACg -3' miRNA: 3'- -GGCUUCUUCCGaCUGG-AGCAGCAC-UG- -5' |
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| 18525 | 5' | -53.1 | NC_004681.1 | + | 68457 | 1.12 | 0.001627 |
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Target: 5'- gCCGAAGAAGGCUGACCUCGUCGUGACg -3' miRNA: 3'- -GGCUUCUUCCGACUGGAGCAGCACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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