Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18526 | 5' | -55.2 | NC_004681.1 | + | 16393 | 0.68 | 0.679588 |
Target: 5'- cGCGGGGaUGCccucggggaGCUGGUagcccuGCGCCUGGu -3' miRNA: 3'- -CGUCCUgAUGaa-------CGACCG------UGCGGACC- -5' |
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18526 | 5' | -55.2 | NC_004681.1 | + | 21394 | 0.68 | 0.679588 |
Target: 5'- -aAGGACgucguggACUUGCaGGagaGCGuCCUGGg -3' miRNA: 3'- cgUCCUGa------UGAACGaCCg--UGC-GGACC- -5' |
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18526 | 5' | -55.2 | NC_004681.1 | + | 48313 | 0.69 | 0.636508 |
Target: 5'- gGCgAGGGCcaGCUUGUaGGCGCGCUucuUGGc -3' miRNA: 3'- -CG-UCCUGa-UGAACGaCCGUGCGG---ACC- -5' |
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18526 | 5' | -55.2 | NC_004681.1 | + | 40020 | 0.69 | 0.593361 |
Target: 5'- gGCGGGcgaaguacucCUGCgcagGCUGGcCGCGCgCUGGg -3' miRNA: 3'- -CGUCCu---------GAUGaa--CGACC-GUGCG-GACC- -5' |
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18526 | 5' | -55.2 | NC_004681.1 | + | 68558 | 1.13 | 0.000805 |
Target: 5'- gGCAGGACUACUUGCUGGCACGCCUGGa -3' miRNA: 3'- -CGUCCUGAUGAACGACCGUGCGGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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