Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18527 | 5' | -59.8 | NC_004681.1 | + | 66836 | 0.66 | 0.522384 |
Target: 5'- --cGGCCUUCUgCGCauUGGCcACCGCGUg -3' miRNA: 3'- cucCUGGGAGA-GCG--ACCGaUGGCGUA- -5' |
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18527 | 5' | -59.8 | NC_004681.1 | + | 68719 | 0.67 | 0.482266 |
Target: 5'- cAGuGACCaugaUCGCUGGC-ACCGCGg -3' miRNA: 3'- cUC-CUGGgag-AGCGACCGaUGGCGUa -5' |
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18527 | 5' | -59.8 | NC_004681.1 | + | 27609 | 0.68 | 0.406923 |
Target: 5'- uGGGucACCCUCgguggCGCUGGCgGCgGCGg -3' miRNA: 3'- cUCC--UGGGAGa----GCGACCGaUGgCGUa -5' |
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18527 | 5' | -59.8 | NC_004681.1 | + | 32717 | 0.69 | 0.331555 |
Target: 5'- uGAGGACCuCUUcCGCUGGCaGCCuGUGUg -3' miRNA: 3'- -CUCCUGG-GAGaGCGACCGaUGG-CGUA- -5' |
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18527 | 5' | -59.8 | NC_004681.1 | + | 68841 | 0.93 | 0.006262 |
Target: 5'- gGAGGACCCUCUCGCUGGCUA-CGCAUg -3' miRNA: 3'- -CUCCUGGGAGAGCGACCGAUgGCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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