miRNA display CGI


Results 1 - 16 of 16 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
18532 5' -52.6 NC_004681.1 + 39402 0.66 0.91443
Target:  5'- cCGCGCGCGcAGguauuugucGAUGGuGG-CCGCg -3'
miRNA:   3'- aGCGCGUGC-UCaa-------CUACCuUCaGGUG- -5'
18532 5' -52.6 NC_004681.1 + 13822 0.66 0.91443
Target:  5'- aUCGUGUACGGGggacUGGAcgauaAGUCCGu -3'
miRNA:   3'- -AGCGCGUGCUCaacuACCU-----UCAGGUg -5'
18532 5' -52.6 NC_004681.1 + 74477 0.66 0.91443
Target:  5'- -aGCGCuuCGAGU--GUGGGAGUCggaGCg -3'
miRNA:   3'- agCGCGu-GCUCAacUACCUUCAGg--UG- -5'
18532 5' -52.6 NC_004681.1 + 70347 0.66 0.901313
Target:  5'- gUGCGCGCGAGccaagugUGGUGGGacgguaacAGUCUc- -3'
miRNA:   3'- aGCGCGUGCUCa------ACUACCU--------UCAGGug -5'
18532 5' -52.6 NC_004681.1 + 10310 0.66 0.89435
Target:  5'- cUCGCGCGCGAGUacc----AGUCCAa -3'
miRNA:   3'- -AGCGCGUGCUCAacuaccuUCAGGUg -5'
18532 5' -52.6 NC_004681.1 + 10396 0.67 0.879636
Target:  5'- cUCGCGCGCGAGgcGaAUGGcgacGUCgAUc -3'
miRNA:   3'- -AGCGCGUGCUCaaC-UACCuu--CAGgUG- -5'
18532 5' -52.6 NC_004681.1 + 42116 0.67 0.879636
Target:  5'- gCGCGCACGAGgu--UGGc-GUCgACa -3'
miRNA:   3'- aGCGCGUGCUCaacuACCuuCAGgUG- -5'
18532 5' -52.6 NC_004681.1 + 28055 0.67 0.878874
Target:  5'- -gGCGguCGAGgcGGUGGcggcaagAAGUCCAa -3'
miRNA:   3'- agCGCguGCUCaaCUACC-------UUCAGGUg -5'
18532 5' -52.6 NC_004681.1 + 71592 0.67 0.855677
Target:  5'- aCGCGCGCGAGUaUGAcccGGGAcagggcaccguGUCUGCc -3'
miRNA:   3'- aGCGCGUGCUCA-ACUa--CCUU-----------CAGGUG- -5'
18532 5' -52.6 NC_004681.1 + 60655 0.68 0.833207
Target:  5'- aUGCGCGCGGGcacuaaacgcgccgcUcgUGAUGGugcGGUCUACg -3'
miRNA:   3'- aGCGCGUGCUC---------------A--ACUACCu--UCAGGUG- -5'
18532 5' -52.6 NC_004681.1 + 39938 0.68 0.801701
Target:  5'- gCGCGCGCGGGcgcGGUgcgcgcGGugcGGUCCACg -3'
miRNA:   3'- aGCGCGUGCUCaa-CUA------CCu--UCAGGUG- -5'
18532 5' -52.6 NC_004681.1 + 50095 0.69 0.741384
Target:  5'- -gGCGCGCGGGaUGGUGGuc-UCCAg -3'
miRNA:   3'- agCGCGUGCUCaACUACCuucAGGUg -5'
18532 5' -52.6 NC_004681.1 + 32029 0.72 0.589374
Target:  5'- aCGUGCAgGAGUUGAggaucgGGAugaGGcCCGCg -3'
miRNA:   3'- aGCGCGUgCUCAACUa-----CCU---UCaGGUG- -5'
18532 5' -52.6 NC_004681.1 + 29397 0.74 0.463059
Target:  5'- -gGUGCGagugGAGUUGAcGGGAGUCCAUg -3'
miRNA:   3'- agCGCGUg---CUCAACUaCCUUCAGGUG- -5'
18532 5' -52.6 NC_004681.1 + 72237 0.75 0.424093
Target:  5'- cCGCGCgGCG-GUUGAUGGAc-UCCACg -3'
miRNA:   3'- aGCGCG-UGCuCAACUACCUucAGGUG- -5'
18532 5' -52.6 NC_004681.1 + 72172 1.1 0.002395
Target:  5'- aUCGCGCACGAGUUGAUGGAAGUCCACc -3'
miRNA:   3'- -AGCGCGUGCUCAACUACCUUCAGGUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.