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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18533 | 3' | -52.2 | NC_004681.1 | + | 58194 | 0.66 | 0.870697 |
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Target: 5'- cGGGCGAGUGUcucaGGUUCguccugagcguGGAGAGgCCg -3' miRNA: 3'- aUCCGUUCACAa---CCGAG-----------UCUUUC-GGa -5' |
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| 18533 | 3' | -52.2 | NC_004681.1 | + | 59814 | 0.66 | 0.863277 |
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Target: 5'- cAGGUuccGAGUGUUGGCaggccgcacucccagCAGggGGCg- -3' miRNA: 3'- aUCCG---UUCACAACCGa--------------GUCuuUCGga -5' |
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| 18533 | 3' | -52.2 | NC_004681.1 | + | 17079 | 0.67 | 0.817528 |
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Target: 5'- cGGuGCcGGUGgauucugGGCUCAGAuGGGCCa -3' miRNA: 3'- aUC-CGuUCACaa-----CCGAGUCU-UUCGGa -5' |
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| 18533 | 3' | -52.2 | NC_004681.1 | + | 58763 | 0.73 | 0.481169 |
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Target: 5'- cAGGCAGGUGUggcccgcGGCgCAGgcGGCCg -3' miRNA: 3'- aUCCGUUCACAa------CCGaGUCuuUCGGa -5' |
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| 18533 | 3' | -52.2 | NC_004681.1 | + | 72792 | 1.06 | 0.003637 |
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Target: 5'- cUAGGCAAGUGUUGGCUCAGAAAGCCUc -3' miRNA: 3'- -AUCCGUUCACAACCGAGUCUUUCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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