Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18533 | 5' | -57.3 | NC_004681.1 | + | 35887 | 0.67 | 0.621599 |
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Target: 5'- -gGGAUGCacGGGcUCCAUCCUCgaaucccGCCCg -3' miRNA: 3'- caUCUACG--CUC-AGGUAGGGGa------CGGGa -5' |
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| 18533 | 5' | -57.3 | NC_004681.1 | + | 36339 | 0.67 | 0.621599 |
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Target: 5'- aGUGGc--CGAGUUCAUagCCUCUGCCCg -3' miRNA: 3'- -CAUCuacGCUCAGGUA--GGGGACGGGa -5' |
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| 18533 | 5' | -57.3 | NC_004681.1 | + | 25586 | 0.69 | 0.494748 |
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Target: 5'- -gAGGUGUccaccaagGAGUUCGUCCCCaccgaaggccccaUGCCCUc -3' miRNA: 3'- caUCUACG--------CUCAGGUAGGGG-------------ACGGGA- -5' |
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| 18533 | 5' | -57.3 | NC_004681.1 | + | 43884 | 0.7 | 0.437137 |
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Target: 5'- cGUGGAaG-GAGgcaaAUCCCCUGCCCUg -3' miRNA: 3'- -CAUCUaCgCUCagg-UAGGGGACGGGA- -5' |
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| 18533 | 5' | -57.3 | NC_004681.1 | + | 33258 | 0.7 | 0.427756 |
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Target: 5'- --uGAUGCGGGUCCucgcaguUCCgCUGCCg- -3' miRNA: 3'- cauCUACGCUCAGGu------AGGgGACGGga -5' |
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| 18533 | 5' | -57.3 | NC_004681.1 | + | 29463 | 0.72 | 0.32516 |
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Target: 5'- -gGGcgGUGAGUCgGUCaCCCUGuCCCUc -3' miRNA: 3'- caUCuaCGCUCAGgUAG-GGGAC-GGGA- -5' |
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| 18533 | 5' | -57.3 | NC_004681.1 | + | 10030 | 0.73 | 0.280465 |
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Target: 5'- cGUGGAcccguacUGCGGGUCaucgCUCCUGCCCa -3' miRNA: 3'- -CAUCU-------ACGCUCAGgua-GGGGACGGGa -5' |
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| 18533 | 5' | -57.3 | NC_004681.1 | + | 72828 | 1.08 | 0.000963 |
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Target: 5'- cGUAGAUGCGAGUCCAUCCCCUGCCCUa -3' miRNA: 3'- -CAUCUACGCUCAGGUAGGGGACGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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