miRNA: 3'- aguGUGGUGACGCCCAUa---GaC-CGUCu -5'
miRNA: 3'- -AGUGugGUGACGcCCAuAGaCCGUcu -5'
miRNA: 3'- agUGUGGU-GACGCCCaUAGaCCGU-CU- -5'
miRNA: 3'- -AGUGUGGUGACGCCCAUAGACCGUCU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18539 | 5' | -55.1 | NC_004682.1 | + | 39717 | 0.66 | 0.694789 |
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Target: 5'- --gUACCGCUGCGGGUAgaacCcGaGCAGu -3' miRNA: 3'- aguGUGGUGACGCCCAUa---GaC-CGUCu -5' |
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| 18539 | 5' | -55.1 | NC_004682.1 | + | 15044 | 0.66 | 0.694789 |
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Target: 5'- uUCGCugCGCUGCcGGU-UCgGGCAa- -3' miRNA: 3'- -AGUGugGUGACGcCCAuAGaCCGUcu -5' |
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| 18539 | 5' | -55.1 | NC_004682.1 | + | 15163 | 0.67 | 0.605798 |
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Target: 5'- -gACGCCAcCUGUGGGcAUCaGGCGuGAa -3' miRNA: 3'- agUGUGGU-GACGCCCaUAGaCCGU-CU- -5' |
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| 18539 | 5' | -55.1 | NC_004682.1 | + | 50640 | 1.09 | 0.000839 |
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Target: 5'- gUCACACCACUGCGGGUAUCUGGCAGAc -3' miRNA: 3'- -AGUGUGGUGACGCCCAUAGACCGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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