Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18540 | 3' | -56.3 | NC_004682.1 | + | 46482 | 0.66 | 0.681766 |
Target: 5'- gCUCACGG-UCUgggcgaAGCCUGCGauccaggCGCGGc -3' miRNA: 3'- aGAGUGUCuAGG------UCGGGCGCa------GUGCC- -5' |
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18540 | 3' | -56.3 | NC_004682.1 | + | 25608 | 0.66 | 0.681766 |
Target: 5'- cCUUGCcGGucUCCGGCUCGCGccgUACGGu -3' miRNA: 3'- aGAGUGuCU--AGGUCGGGCGCa--GUGCC- -5' |
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18540 | 3' | -56.3 | NC_004682.1 | + | 40437 | 0.66 | 0.660095 |
Target: 5'- cUCUCGuugGGGUCCAGCuCCGgGUgGUGGa -3' miRNA: 3'- -AGAGUg--UCUAGGUCG-GGCgCAgUGCC- -5' |
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18540 | 3' | -56.3 | NC_004682.1 | + | 46926 | 0.67 | 0.616525 |
Target: 5'- --cUACAGAUCaaugaGGCCCGcCGaaGCGGg -3' miRNA: 3'- agaGUGUCUAGg----UCGGGC-GCagUGCC- -5' |
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18540 | 3' | -56.3 | NC_004682.1 | + | 8856 | 0.67 | 0.594782 |
Target: 5'- -gUCcgAGcgCCGGCCCgacgccguagGCGUCACGGu -3' miRNA: 3'- agAGugUCuaGGUCGGG----------CGCAGUGCC- -5' |
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18540 | 3' | -56.3 | NC_004682.1 | + | 41549 | 0.68 | 0.541108 |
Target: 5'- cCUCGCGGuUCCAGaugaCCGUGUUguuCGGc -3' miRNA: 3'- aGAGUGUCuAGGUCg---GGCGCAGu--GCC- -5' |
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18540 | 3' | -56.3 | NC_004682.1 | + | 26249 | 0.68 | 0.520073 |
Target: 5'- gUCggagCACGacaCCAGCaCCGCGuUCGCGGg -3' miRNA: 3'- -AGa---GUGUcuaGGUCG-GGCGC-AGUGCC- -5' |
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18540 | 3' | -56.3 | NC_004682.1 | + | 39596 | 0.69 | 0.449324 |
Target: 5'- cUUCACGaccUCAGCCCGCGUaGCGGu -3' miRNA: 3'- aGAGUGUcuaGGUCGGGCGCAgUGCC- -5' |
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18540 | 3' | -56.3 | NC_004682.1 | + | 50230 | 1.11 | 0.000548 |
Target: 5'- aUCUCACAGAUCCAGCCCGCGUCACGGa -3' miRNA: 3'- -AGAGUGUCUAGGUCGGGCGCAGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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