Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18540 | 5' | -59.1 | NC_004682.1 | + | 3166 | 0.7 | 0.308168 |
Target: 5'- aCUCgGgcaccacGGCGGGCCAGggCG-CGGGc -3' miRNA: 3'- -GAGgCaua----CCGCCCGGUCa-GCaGCCC- -5' |
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18540 | 5' | -59.1 | NC_004682.1 | + | 22977 | 0.67 | 0.452596 |
Target: 5'- cCUCCGggcUGGCcuucGGGCUGGUC--CGGGg -3' miRNA: 3'- -GAGGCau-ACCG----CCCGGUCAGcaGCCC- -5' |
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18540 | 5' | -59.1 | NC_004682.1 | + | 23680 | 0.66 | 0.521875 |
Target: 5'- -gCCGa---GCaGGGCCacacGGUCGUCGGGu -3' miRNA: 3'- gaGGCauacCG-CCCGG----UCAGCAGCCC- -5' |
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18540 | 5' | -59.1 | NC_004682.1 | + | 37545 | 0.67 | 0.462196 |
Target: 5'- gCUCCac----CGGGCCGG-CGUCGGGu -3' miRNA: 3'- -GAGGcauaccGCCCGGUCaGCAGCCC- -5' |
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18540 | 5' | -59.1 | NC_004682.1 | + | 42636 | 0.67 | 0.485662 |
Target: 5'- -cCCGgcacgccacaagGGCGGuCCAGUCGUUGGa -3' miRNA: 3'- gaGGCaua---------CCGCCcGGUCAGCAGCCc -5' |
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18540 | 5' | -59.1 | NC_004682.1 | + | 45560 | 0.67 | 0.452596 |
Target: 5'- -aCCGU-UGGCGuacuccucgguGGCCcggaAGUCGUCGGa -3' miRNA: 3'- gaGGCAuACCGC-----------CCGG----UCAGCAGCCc -5' |
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18540 | 5' | -59.1 | NC_004682.1 | + | 50265 | 1.09 | 0.00046 |
Target: 5'- uCUCCGUAUGGCGGGCCAGUCGUCGGGu -3' miRNA: 3'- -GAGGCAUACCGCCCGGUCAGCAGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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