Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18541 | 5' | -56.4 | NC_004682.1 | + | 41650 | 0.66 | 0.654861 |
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Target: 5'- uGUCgggugCgCGGGUCGAUCUGuGcGUaCAGGu -3' miRNA: 3'- -CAGaa---G-GCCCAGCUAGAC-C-CAgGUCC- -5' |
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| 18541 | 5' | -56.4 | NC_004682.1 | + | 18111 | 0.67 | 0.611376 |
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Target: 5'- aUCUacgCCGaGGcCGGUCUGGGUCggaagaAGGc -3' miRNA: 3'- cAGAa--GGC-CCaGCUAGACCCAGg-----UCC- -5' |
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| 18541 | 5' | -56.4 | NC_004682.1 | + | 18180 | 0.67 | 0.600526 |
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Target: 5'- ---cUCUGGG-CGAUCUGGGcucguUCCuGGu -3' miRNA: 3'- cagaAGGCCCaGCUAGACCC-----AGGuCC- -5' |
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| 18541 | 5' | -56.4 | NC_004682.1 | + | 26400 | 0.67 | 0.600526 |
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Target: 5'- cGUCg-CCGGGUCGAacgUCggcgGGG-CCAGc -3' miRNA: 3'- -CAGaaGGCCCAGCU---AGa---CCCaGGUCc -5' |
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| 18541 | 5' | -56.4 | NC_004682.1 | + | 35475 | 0.67 | 0.568167 |
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Target: 5'- cUCguagUCCGGGU-GGUCcGGGUCgCAGa -3' miRNA: 3'- cAGa---AGGCCCAgCUAGaCCCAG-GUCc -5' |
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| 18541 | 5' | -56.4 | NC_004682.1 | + | 25648 | 0.68 | 0.515342 |
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Target: 5'- ---gUCCGGG-CGcUCcacugGGGUCCGGGu -3' miRNA: 3'- cagaAGGCCCaGCuAGa----CCCAGGUCC- -5' |
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| 18541 | 5' | -56.4 | NC_004682.1 | + | 37822 | 0.71 | 0.388416 |
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Target: 5'- gGUCUucgagguagUCCGGGUCGGUgaccUUGguggccaGGUCCGGGa -3' miRNA: 3'- -CAGA---------AGGCCCAGCUA----GAC-------CCAGGUCC- -5' |
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| 18541 | 5' | -56.4 | NC_004682.1 | + | 25348 | 0.77 | 0.160712 |
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Target: 5'- cUCUUCggaGGGUgGAUCUGGGUCUGGa -3' miRNA: 3'- cAGAAGg--CCCAgCUAGACCCAGGUCc -5' |
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| 18541 | 5' | -56.4 | NC_004682.1 | + | 47240 | 1.1 | 0.000737 |
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Target: 5'- cGUCUUCCGGGUCGAUCUGGGUCCAGGc -3' miRNA: 3'- -CAGAAGGCCCAGCUAGACCCAGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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