miRNA: 3'- aGCaa----GCAGUCCAUCu-----------GGCUGCACau -5'
miRNA: 3'- aGCaaGCAGUCC--------------A--UCUGGCUGCACAu -5'
miRNA: 3'- -AGcAAGCAGUcCAUCUGGCUGCACau -5'
miRNA: 3'- -AGCAag--CAGUCCaUCUGGCUGCACAU- -5'
miRNA: 3'- -AGCAAGCAGUCCAUCUGGCUGCACAU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18543 | 3' | -52.1 | NC_004682.1 | + | 37551 | 0.66 | 0.870481 |
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Target: 5'- cCGggccggCGUCGGGUAGuccaucaggucuCCGAUGUGg- -3' miRNA: 3'- aGCaa----GCAGUCCAUCu-----------GGCUGCACau -5' |
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| 18543 | 3' | -52.1 | NC_004682.1 | + | 28256 | 0.66 | 0.852901 |
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Target: 5'- cCGaaCGUCAGGaagccucaucggcuUcgGGGCCGGCGUGUc -3' miRNA: 3'- aGCaaGCAGUCC--------------A--UCUGGCUGCACAu -5' |
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| 18543 | 3' | -52.1 | NC_004682.1 | + | 37924 | 0.7 | 0.660797 |
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Target: 5'- uUC-UUCGUCA-GUGGGCUGACGUGc- -3' miRNA: 3'- -AGcAAGCAGUcCAUCUGGCUGCACau -5' |
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| 18543 | 3' | -52.1 | NC_004682.1 | + | 35612 | 0.74 | 0.414116 |
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Target: 5'- uUCGUgaaaGUUGGGcGGGCCGAUGUGUGa -3' miRNA: 3'- -AGCAag--CAGUCCaUCUGGCUGCACAU- -5' |
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| 18543 | 3' | -52.1 | NC_004682.1 | + | 46093 | 1.08 | 0.002366 |
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Target: 5'- aUCGUUCGUCAGGUAGACCGACGUGUAg -3' miRNA: 3'- -AGCAAGCAGUCCAUCUGGCUGCACAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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