Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18544 | 5' | -57.8 | NC_004682.1 | + | 10875 | 0.66 | 0.513385 |
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Target: 5'- gUUGUggaUCGACGcAGGaCGCGAagcguCCCGGUUg -3' miRNA: 3'- -AACA---AGCUGCcUCC-GCGCU-----GGGUCAA- -5' |
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| 18544 | 5' | -57.8 | NC_004682.1 | + | 17070 | 0.66 | 0.512332 |
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Target: 5'- ----aCGACGGgcaugcaGGGCGUGGCUCAGg- -3' miRNA: 3'- aacaaGCUGCC-------UCCGCGCUGGGUCaa -5' |
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| 18544 | 5' | -57.8 | NC_004682.1 | + | 47529 | 0.67 | 0.45198 |
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Target: 5'- cUGggUCGACGG-GGUGCGuGCCCAu-- -3' miRNA: 3'- aACa-AGCUGCCuCCGCGC-UGGGUcaa -5' |
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| 18544 | 5' | -57.8 | NC_004682.1 | + | 10508 | 0.69 | 0.359387 |
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Target: 5'- ----gUGACGcAGGCGUGGCCCAGg- -3' miRNA: 3'- aacaaGCUGCcUCCGCGCUGGGUCaa -5' |
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| 18544 | 5' | -57.8 | NC_004682.1 | + | 8121 | 0.74 | 0.169969 |
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Target: 5'- -cGUUccCGACgcuGGAGGcCGCGACCCGGUUc -3' miRNA: 3'- aaCAA--GCUG---CCUCC-GCGCUGGGUCAA- -5' |
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| 18544 | 5' | -57.8 | NC_004682.1 | + | 45988 | 1.02 | 0.001347 |
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Target: 5'- cUUGUUCGACGGAGGCGCGACCCAGUUc -3' miRNA: 3'- -AACAAGCUGCCUCCGCGCUGGGUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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