Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18545 | 3' | -52.4 | NC_004682.1 | + | 22827 | 0.66 | 0.861272 |
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Target: 5'- gAGGUGAUCUGAGugaagaucUCCGGGcucaucGUGgagCUg -3' miRNA: 3'- gUCCACUAGACUU--------AGGUCCa-----CGCa--GG- -5' |
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| 18545 | 3' | -52.4 | NC_004682.1 | + | 27357 | 0.66 | 0.852746 |
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Target: 5'- -uGGUGAUCgccgGAAgcucgaacaucUCCcGGacgGCGUCCu -3' miRNA: 3'- guCCACUAGa---CUU-----------AGGuCCa--CGCAGG- -5' |
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| 18545 | 3' | -52.4 | NC_004682.1 | + | 6525 | 0.66 | 0.825747 |
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Target: 5'- -cGGUGGUCgucggCCAGGUGC-UCa -3' miRNA: 3'- guCCACUAGacuuaGGUCCACGcAGg -5' |
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| 18545 | 3' | -52.4 | NC_004682.1 | + | 21198 | 0.66 | 0.825747 |
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Target: 5'- -uGGUGGcgCUGAAg-CAGG-GCGUCUg -3' miRNA: 3'- guCCACUa-GACUUagGUCCaCGCAGG- -5' |
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| 18545 | 3' | -52.4 | NC_004682.1 | + | 19670 | 0.67 | 0.78681 |
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Target: 5'- aGGGUGGUCUGAAUCCGac--CGaCCa -3' miRNA: 3'- gUCCACUAGACUUAGGUccacGCaGG- -5' |
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| 18545 | 3' | -52.4 | NC_004682.1 | + | 20756 | 0.68 | 0.733353 |
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Target: 5'- -cGGUGGUCcGggUucauggaucacuaCCGGGUG-GUCCg -3' miRNA: 3'- guCCACUAGaCuuA-------------GGUCCACgCAGG- -5' |
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| 18545 | 3' | -52.4 | NC_004682.1 | + | 29846 | 0.68 | 0.712642 |
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Target: 5'- gGGGUcGAgcgGGuuGUCCGGGaGCGUCCa -3' miRNA: 3'- gUCCA-CUagaCU--UAGGUCCaCGCAGG- -5' |
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| 18545 | 3' | -52.4 | NC_004682.1 | + | 47246 | 0.7 | 0.611825 |
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Target: 5'- cCGGGUcGAUCUGggUCCAGGccacgguagGCacGUaCCg -3' miRNA: 3'- -GUCCA-CUAGACuuAGGUCCa--------CG--CA-GG- -5' |
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| 18545 | 3' | -52.4 | NC_004682.1 | + | 45619 | 0.71 | 0.54492 |
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Target: 5'- gCAGG-GA-CUGucUCCGGGUGUaGUCCg -3' miRNA: 3'- -GUCCaCUaGACuuAGGUCCACG-CAGG- -5' |
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| 18545 | 3' | -52.4 | NC_004682.1 | + | 45503 | 1.11 | 0.001257 |
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Target: 5'- cCAGGUGAUCUGAAUCCAGGUGCGUCCc -3' miRNA: 3'- -GUCCACUAGACUUAGGUCCACGCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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