Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18545 | 5' | -61.8 | NC_004682.1 | + | 48887 | 0.67 | 0.339914 |
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Target: 5'- gCCAGCGaUCCaGaUCCGccaucGGGGACCGa -3' miRNA: 3'- -GGUCGCgAGGaCcAGGU-----CCCCUGGCa -5' |
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| 18545 | 5' | -61.8 | NC_004682.1 | + | 25650 | 0.68 | 0.288033 |
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Target: 5'- cCgGGCGCUCCacuggGGUCCGGGuaccaACCGg -3' miRNA: 3'- -GgUCGCGAGGa----CCAGGUCCcc---UGGCa -5' |
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| 18545 | 5' | -61.8 | NC_004682.1 | + | 50463 | 0.68 | 0.281151 |
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Target: 5'- -gAGCGCUCCgGGcgcUCCcggAGGGGAgCCGc -3' miRNA: 3'- ggUCGCGAGGaCC---AGG---UCCCCU-GGCa -5' |
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| 18545 | 5' | -61.8 | NC_004682.1 | + | 41946 | 0.68 | 0.274401 |
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Target: 5'- gCgAGCaGCUUCUGcaucugCUGGGGGACCGUg -3' miRNA: 3'- -GgUCG-CGAGGACca----GGUCCCCUGGCA- -5' |
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| 18545 | 5' | -61.8 | NC_004682.1 | + | 43146 | 0.69 | 0.254928 |
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Target: 5'- gCCA-CGCUCC-GGUCCAGGcGGucgucCCGg -3' miRNA: 3'- -GGUcGCGAGGaCCAGGUCC-CCu----GGCa -5' |
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| 18545 | 5' | -61.8 | NC_004682.1 | + | 21912 | 0.72 | 0.160515 |
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Target: 5'- gUCAGCGUUCCUGGcgaCCAGGGccaagaucugggaGACCa- -3' miRNA: 3'- -GGUCGCGAGGACCa--GGUCCC-------------CUGGca -5' |
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| 18545 | 5' | -61.8 | NC_004682.1 | + | 22438 | 0.72 | 0.152677 |
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Target: 5'- aCGGCGCUCCUGcgggccGUCCAGGGuGCaGUa -3' miRNA: 3'- gGUCGCGAGGAC------CAGGUCCCcUGgCA- -5' |
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| 18545 | 5' | -61.8 | NC_004682.1 | + | 2634 | 0.72 | 0.141007 |
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Target: 5'- gCCGGCG-UCCUGGUCgAGacauGGGACCa- -3' miRNA: 3'- -GGUCGCgAGGACCAGgUC----CCCUGGca -5' |
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| 18545 | 5' | -61.8 | NC_004682.1 | + | 22984 | 0.77 | 0.069492 |
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Target: 5'- gCUGGC-CUUcgggCUGGUCCGGGGGGCCGUc -3' miRNA: 3'- -GGUCGcGAG----GACCAGGUCCCCUGGCA- -5' |
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| 18545 | 5' | -61.8 | NC_004682.1 | + | 45538 | 1.07 | 0.000298 |
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Target: 5'- uCCAGCGCUCCUGGUCCAGGGGACCGUu -3' miRNA: 3'- -GGUCGCGAGGACCAGGUCCCCUGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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