Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18546 | 3' | -58.2 | NC_004682.1 | + | 5462 | 0.66 | 0.482482 |
Target: 5'- --aGUgGGCCgGCaUCGGcgCGGCCu -3' miRNA: 3'- cuaCGgCCGGgUGaAGUCuaGCCGGu -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 6114 | 0.73 | 0.17632 |
Target: 5'- cGAUGCUGGCCCGCUaCg---CGGCCu -3' miRNA: 3'- -CUACGGCCGGGUGAaGucuaGCCGGu -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 6894 | 0.66 | 0.489623 |
Target: 5'- --aGCUGGCCCGCgucgacaagguauggCAGA--GGCCAg -3' miRNA: 3'- cuaCGGCCGGGUGaa-------------GUCUagCCGGU- -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 14680 | 0.66 | 0.503012 |
Target: 5'- --cGCCGaCCgGCUgggaCAGcAUCGGCCAc -3' miRNA: 3'- cuaCGGCcGGgUGAa---GUC-UAGCCGGU- -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 16521 | 0.7 | 0.282351 |
Target: 5'- gGAUGCUGGCCgACUaCGGG-CGGCa- -3' miRNA: 3'- -CUACGGCCGGgUGAaGUCUaGCCGgu -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 24927 | 0.67 | 0.452473 |
Target: 5'- cGGUGCUGGCCCccGCUUCGca-CaGCCAa -3' miRNA: 3'- -CUACGGCCGGG--UGAAGUcuaGcCGGU- -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 27451 | 0.66 | 0.523914 |
Target: 5'- aGUGCCGaGCCagcCUUCaAGcUCGGCCc -3' miRNA: 3'- cUACGGC-CGGgu-GAAG-UCuAGCCGGu -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 28696 | 0.68 | 0.414107 |
Target: 5'- cGAUGCC-GCCCACUcgCGGcggCGGCgGg -3' miRNA: 3'- -CUACGGcCGGGUGAa-GUCua-GCCGgU- -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 31138 | 0.67 | 0.433043 |
Target: 5'- --aGCCaGGCagaauCCACUUCagccAGAUCGGCUg -3' miRNA: 3'- cuaCGG-CCG-----GGUGAAG----UCUAGCCGGu -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 32216 | 0.67 | 0.442698 |
Target: 5'- -cUGCgCGGCCagcuuuCGCUUCAGcUCGGCg- -3' miRNA: 3'- cuACG-GCCGG------GUGAAGUCuAGCCGgu -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 32926 | 0.69 | 0.327514 |
Target: 5'- aGGUGCCGGgaCGCUcCAGcUCGGUCAg -3' miRNA: 3'- -CUACGGCCggGUGAaGUCuAGCCGGU- -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 33154 | 0.69 | 0.31963 |
Target: 5'- cGGUGCCGGCCCAguagggauCUUCgAGGaCGGUgGa -3' miRNA: 3'- -CUACGGCCGGGU--------GAAG-UCUaGCCGgU- -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 34869 | 0.68 | 0.404833 |
Target: 5'- --aGCCGGCCUug--CGGAUCuGCCGu -3' miRNA: 3'- cuaCGGCCGGGugaaGUCUAGcCGGU- -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 36984 | 0.71 | 0.255075 |
Target: 5'- cGAUGCgCGGgCCugUgcCAGGUUGGCCu -3' miRNA: 3'- -CUACG-GCCgGGugAa-GUCUAGCCGGu -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 38565 | 0.67 | 0.442698 |
Target: 5'- gGAUG-CGGCCCuca-CGGAUCGcGCCGa -3' miRNA: 3'- -CUACgGCCGGGugaaGUCUAGC-CGGU- -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 38682 | 0.69 | 0.36048 |
Target: 5'- gGGUGCCGGUCCACUUCuu----GCCGc -3' miRNA: 3'- -CUACGGCCGGGUGAAGucuagcCGGU- -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 38781 | 0.66 | 0.523914 |
Target: 5'- uGGUGUCGGCCagcgaCACgaccuuggUCGucUCGGCCAg -3' miRNA: 3'- -CUACGGCCGG-----GUGa-------AGUcuAGCCGGU- -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 44511 | 0.66 | 0.523914 |
Target: 5'- gGAgucCCGGaCCCAg--CGGGUCGGCCu -3' miRNA: 3'- -CUac-GGCC-GGGUgaaGUCUAGCCGGu -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 44564 | 0.68 | 0.369075 |
Target: 5'- aGAUGCCGcuuuCCCGCUcuUCGGGggUGGCCu -3' miRNA: 3'- -CUACGGCc---GGGUGA--AGUCUa-GCCGGu -5' |
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18546 | 3' | -58.2 | NC_004682.1 | + | 45388 | 1.08 | 0.000442 |
Target: 5'- cGAUGCCGGCCCACUUCAGAUCGGCCAg -3' miRNA: 3'- -CUACGGCCGGGUGAAGUCUAGCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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