Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18546 | 5' | -54.1 | NC_004682.1 | + | 18888 | 0.66 | 0.799022 |
Target: 5'- -aUGGCGAGCugGgacUCGcuGGUCGaGUCGg -3' miRNA: 3'- gaACUGCUUGugC---AGCc-CCAGC-CAGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 10373 | 0.66 | 0.799022 |
Target: 5'- -aUGACGggUuccgaGUCGGGGUCucgcaGGUa- -3' miRNA: 3'- gaACUGCuuGug---CAGCCCCAG-----CCAgu -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 24517 | 0.66 | 0.789272 |
Target: 5'- --aGACGuACAcCGccaacccaaugcUCGGGGUCGGcCAc -3' miRNA: 3'- gaaCUGCuUGU-GC------------AGCCCCAGCCaGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 43222 | 0.66 | 0.789272 |
Target: 5'- -aUGACGA--ACGUCGGG--CGGUUAc -3' miRNA: 3'- gaACUGCUugUGCAGCCCcaGCCAGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 42143 | 0.66 | 0.779359 |
Target: 5'- --cGAUGAGCGCGUCGuGGaUCGcUCGg -3' miRNA: 3'- gaaCUGCUUGUGCAGC-CCcAGCcAGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 36904 | 0.66 | 0.769292 |
Target: 5'- gUUGAUGAACGaGUCGacaucgcucGGGUgaCGGUCAc -3' miRNA: 3'- gAACUGCUUGUgCAGC---------CCCA--GCCAGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 10558 | 0.66 | 0.759084 |
Target: 5'- -aUGGCaGGACGCcgacagaucaUCGGGG-CGGUCAa -3' miRNA: 3'- gaACUG-CUUGUGc---------AGCCCCaGCCAGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 38049 | 0.68 | 0.6955 |
Target: 5'- --cGAC-AGCGCGUUcaGuGGGUCGGUCc -3' miRNA: 3'- gaaCUGcUUGUGCAG--C-CCCAGCCAGu -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 43111 | 0.69 | 0.607656 |
Target: 5'- --cGACGGGCAC--CGGGGaucUUGGUCAg -3' miRNA: 3'- gaaCUGCUUGUGcaGCCCC---AGCCAGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 42460 | 0.69 | 0.58571 |
Target: 5'- --gGAUGAACugGcuggcCGGGGUCGGcCu -3' miRNA: 3'- gaaCUGCUUGugCa----GCCCCAGCCaGu -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 35410 | 0.7 | 0.530602 |
Target: 5'- -aUGACGAagGCACcggcccaGUCGGGGUCGuacuccGUCGa -3' miRNA: 3'- gaACUGCU--UGUG-------CAGCCCCAGC------CAGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 49261 | 0.71 | 0.500103 |
Target: 5'- --cGAUccggGAugGCGUCGGcGUCGGUCGa -3' miRNA: 3'- gaaCUG----CUugUGCAGCCcCAGCCAGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 31285 | 0.72 | 0.459376 |
Target: 5'- --cGAguaGAGCugGUaguaGGGGUUGGUCAg -3' miRNA: 3'- gaaCUg--CUUGugCAg---CCCCAGCCAGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 30391 | 0.72 | 0.459376 |
Target: 5'- gCUUGGCGcucagccgcuGCACGcCGGGGUaggccCGGUCGa -3' miRNA: 3'- -GAACUGCu---------UGUGCaGCCCCA-----GCCAGU- -5' |
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18546 | 5' | -54.1 | NC_004682.1 | + | 45427 | 1.08 | 0.001628 |
Target: 5'- uCUUGACGAACACGUCGGGGUCGGUCAc -3' miRNA: 3'- -GAACUGCUUGUGCAGCCCCAGCCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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