Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18547 | 5' | -55.3 | NC_004682.1 | + | 41817 | 0.66 | 0.709794 |
Target: 5'- cUCGCGccAGCAGCGCGUaggUCagGACGaagGCUg -3' miRNA: 3'- cAGCGC--UCGUUGCGCG---AG--CUGCa--UGA- -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 16195 | 0.66 | 0.708714 |
Target: 5'- -aCGCGAGguGCuggaucgcaaccgGCGCaUCGGCGUGg- -3' miRNA: 3'- caGCGCUCguUG-------------CGCG-AGCUGCAUga -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 35207 | 0.67 | 0.65512 |
Target: 5'- --gGCGGGCGA-GCGCUCGGCu---- -3' miRNA: 3'- cagCGCUCGUUgCGCGAGCUGcauga -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 27877 | 0.67 | 0.65512 |
Target: 5'- -aCGCGAGCGccaggggaGCaGCGa-CGACGUACa -3' miRNA: 3'- caGCGCUCGU--------UG-CGCgaGCUGCAUGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 41936 | 0.67 | 0.65181 |
Target: 5'- -cCGUGGGCAGCGgGUcaccggcagagcccUUGAUGUACa -3' miRNA: 3'- caGCGCUCGUUGCgCG--------------AGCUGCAUGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 17522 | 0.67 | 0.641868 |
Target: 5'- cUCGgGccugaucGGCAACGUGCUCGGCucacuggGUACg -3' miRNA: 3'- cAGCgC-------UCGUUGCGCGAGCUG-------CAUGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 10582 | 0.67 | 0.633025 |
Target: 5'- -aCGCu-GCGA-GCGCUCGACGcACUg -3' miRNA: 3'- caGCGcuCGUUgCGCGAGCUGCaUGA- -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 45899 | 0.67 | 0.621971 |
Target: 5'- -gCGCGAGUAGCGCacucggGCUCGcCGU-Cg -3' miRNA: 3'- caGCGCUCGUUGCG------CGAGCuGCAuGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 39661 | 0.68 | 0.567021 |
Target: 5'- --aGCGGGCGGCuucugaGCGgaguccCUCGACGUACUc -3' miRNA: 3'- cagCGCUCGUUG------CGC------GAGCUGCAUGA- -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 11380 | 0.68 | 0.556157 |
Target: 5'- aUCcCGAGCGaagACGUGCUCGAUGU-Cg -3' miRNA: 3'- cAGcGCUCGU---UGCGCGAGCUGCAuGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 9393 | 0.68 | 0.556157 |
Target: 5'- gGUCGaCGAGCGccuGCGUGgUCGGCcUGCa -3' miRNA: 3'- -CAGC-GCUCGU---UGCGCgAGCUGcAUGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 13627 | 0.68 | 0.545355 |
Target: 5'- -aCGCGGGCAGCacgGCcCUCGGCGcgGCg -3' miRNA: 3'- caGCGCUCGUUG---CGcGAGCUGCa-UGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 37724 | 0.69 | 0.523962 |
Target: 5'- cUCGaCGGGCuccucguaGAUGCGCUCGACGcagccUGCg -3' miRNA: 3'- cAGC-GCUCG--------UUGCGCGAGCUGC-----AUGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 40689 | 0.69 | 0.502898 |
Target: 5'- gGUUGCGGaugacccCGGCGCGCUUGcCGUGCUc -3' miRNA: 3'- -CAGCGCUc------GUUGCGCGAGCuGCAUGA- -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 4936 | 0.69 | 0.502898 |
Target: 5'- cGUCGCGuGCAugG-GCUaCGACG-ACa -3' miRNA: 3'- -CAGCGCuCGUugCgCGA-GCUGCaUGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 12956 | 0.69 | 0.502898 |
Target: 5'- uUCGCGGacaucGCGGCGCGUucUCGGgGUACc -3' miRNA: 3'- cAGCGCU-----CGUUGCGCG--AGCUgCAUGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 45768 | 0.69 | 0.492504 |
Target: 5'- -gCGgGAGCAGCcgGUGCggcgUCGGCGUACg -3' miRNA: 3'- caGCgCUCGUUG--CGCG----AGCUGCAUGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 2963 | 0.69 | 0.492504 |
Target: 5'- -gCGCGGGCGGCgGCGCUgGGCaGUGg- -3' miRNA: 3'- caGCGCUCGUUG-CGCGAgCUG-CAUga -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 6365 | 0.69 | 0.482211 |
Target: 5'- -gCGCGGGCG--GUGCUCGACaaGUACa -3' miRNA: 3'- caGCGCUCGUugCGCGAGCUG--CAUGa -5' |
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18547 | 5' | -55.3 | NC_004682.1 | + | 5918 | 0.7 | 0.45198 |
Target: 5'- aGUCGUGGGCGuuccaGCaGCUCGACGgcaaggGCg -3' miRNA: 3'- -CAGCGCUCGUug---CG-CGAGCUGCa-----UGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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