Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18548 | 3' | -55.9 | NC_004682.1 | + | 46127 | 0.66 | 0.643618 |
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Target: 5'- uGCGgcacACGGCGAuCCAGCaccUGCUCGa -3' miRNA: 3'- -CGUacu-UGCCGCUcGGUCGc--ACGAGU- -5' |
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| 18548 | 3' | -55.9 | NC_004682.1 | + | 32977 | 0.66 | 0.610324 |
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Target: 5'- ---aGAACGuCGcGCUGGCGUGCUCc -3' miRNA: 3'- cguaCUUGCcGCuCGGUCGCACGAGu -5' |
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| 18548 | 3' | -55.9 | NC_004682.1 | + | 33226 | 0.67 | 0.599251 |
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Target: 5'- gGCAccAGCaGCGAGcCCAGCGUGUcCGc -3' miRNA: 3'- -CGUacUUGcCGCUC-GGUCGCACGaGU- -5' |
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| 18548 | 3' | -55.9 | NC_004682.1 | + | 41974 | 0.67 | 0.56624 |
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Target: 5'- cCGUGAcgccuACGGCGucgGGCCGGCGcucgGaCUCAu -3' miRNA: 3'- cGUACU-----UGCCGC---UCGGUCGCa---C-GAGU- -5' |
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| 18548 | 3' | -55.9 | NC_004682.1 | + | 3171 | 0.68 | 0.533717 |
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Target: 5'- gGCAcc-ACGGCGGGCCAGgGcGCgggCAa -3' miRNA: 3'- -CGUacuUGCCGCUCGGUCgCaCGa--GU- -5' |
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| 18548 | 3' | -55.9 | NC_004682.1 | + | 6516 | 0.68 | 0.523019 |
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Target: 5'- cGCAaGGcGCGGUGgucgucGGCCAG-GUGCUCAa -3' miRNA: 3'- -CGUaCU-UGCCGC------UCGGUCgCACGAGU- -5' |
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| 18548 | 3' | -55.9 | NC_004682.1 | + | 45566 | 0.69 | 0.478049 |
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Target: 5'- gGCGUGAgcagcagccccagcGCGGCGAGCaUGGCG-GCgaUCAg -3' miRNA: 3'- -CGUACU--------------UGCCGCUCG-GUCGCaCG--AGU- -5' |
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| 18548 | 3' | -55.9 | NC_004682.1 | + | 47099 | 0.75 | 0.193283 |
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Target: 5'- uGCccGGACGGCGuAGUCAGUGaugGCUCAg -3' miRNA: 3'- -CGuaCUUGCCGC-UCGGUCGCa--CGAGU- -5' |
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| 18548 | 3' | -55.9 | NC_004682.1 | + | 43633 | 1.1 | 0.000615 |
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Target: 5'- gGCAUGAACGGCGAGCCAGCGUGCUCAc -3' miRNA: 3'- -CGUACUUGCCGCUCGGUCGCACGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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