Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18549 | 5' | -54.7 | NC_004682.1 | + | 45723 | 0.66 | 0.745248 |
Target: 5'- cGCGcCAGGAUCggcgcggCGUCGACCacGGgGGu -3' miRNA: 3'- cUGUaGUCCUAGa------GCAGCUGG--CCgUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 36034 | 0.66 | 0.745248 |
Target: 5'- aGCGUCAGGua---GUCGACCGGa-- -3' miRNA: 3'- cUGUAGUCCuagagCAGCUGGCCguc -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 22873 | 0.66 | 0.734693 |
Target: 5'- uGGCAUCAuGGccgUCGUCGGCCuGGUGGc -3' miRNA: 3'- -CUGUAGU-CCuagAGCAGCUGG-CCGUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 42949 | 0.66 | 0.734693 |
Target: 5'- uGCGagGGGAUCUCGgaGACCGGgGu -3' miRNA: 3'- cUGUagUCCUAGAGCagCUGGCCgUc -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 34373 | 0.66 | 0.733631 |
Target: 5'- -uCGUCGGcGcAUCUCGUUGACCgacuugaGGUAGg -3' miRNA: 3'- cuGUAGUC-C-UAGAGCAGCUGG-------CCGUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 36339 | 0.66 | 0.724033 |
Target: 5'- -uUAUCAGGAUCUCGgugggCGAaccCCGaGCuGg -3' miRNA: 3'- cuGUAGUCCUAGAGCa----GCU---GGC-CGuC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 35207 | 0.66 | 0.717592 |
Target: 5'- cGC-UCAGGAUCUCGcacacgUCGAUcaacauccgagccaaCGGCAGc -3' miRNA: 3'- cUGuAGUCCUAGAGC------AGCUG---------------GCCGUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 13190 | 0.67 | 0.691545 |
Target: 5'- cGACAcgCAG-AUCcCGUuccaCGACCGGCGGg -3' miRNA: 3'- -CUGUa-GUCcUAGaGCA----GCUGGCCGUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 31371 | 0.67 | 0.691545 |
Target: 5'- -cCAUCAGGGUCUUGaCGuucUCGGCGa -3' miRNA: 3'- cuGUAGUCCUAGAGCaGCu--GGCCGUc -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 13695 | 0.67 | 0.658533 |
Target: 5'- cGGC-UgGGGAUCUCGUCGcACacguucggcuaCGGCGGc -3' miRNA: 3'- -CUGuAgUCCUAGAGCAGC-UG-----------GCCGUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 14313 | 0.68 | 0.636382 |
Target: 5'- gGGCGUgAGGGUCUCGUUgaGACCGa--- -3' miRNA: 3'- -CUGUAgUCCUAGAGCAG--CUGGCcguc -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 18820 | 0.68 | 0.625295 |
Target: 5'- cGAC--CAGG-UCUcCG-CGACCGGCAGu -3' miRNA: 3'- -CUGuaGUCCuAGA-GCaGCUGGCCGUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 42258 | 0.68 | 0.60315 |
Target: 5'- uGGCGUCGuagguaguuGGAUgUCGUCGugCGuuGCAGa -3' miRNA: 3'- -CUGUAGU---------CCUAgAGCAGCugGC--CGUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 33192 | 0.68 | 0.592111 |
Target: 5'- gGGCGUCAGGAUgUUGcCGAgCGucuGCAGg -3' miRNA: 3'- -CUGUAGUCCUAgAGCaGCUgGC---CGUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 41746 | 0.69 | 0.548382 |
Target: 5'- --gGUCAGGAUCgacagCGACCGGgAGa -3' miRNA: 3'- cugUAGUCCUAGagca-GCUGGCCgUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 18922 | 0.69 | 0.537597 |
Target: 5'- cGAUGUCGGGAUCUCcggCGA-CGGCGc -3' miRNA: 3'- -CUGUAGUCCUAGAGca-GCUgGCCGUc -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 20263 | 0.72 | 0.361334 |
Target: 5'- cGCGUguGGAgcaggUCUCGUCGGagaaCGGCAGc -3' miRNA: 3'- cUGUAguCCU-----AGAGCAGCUg---GCCGUC- -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 34291 | 0.73 | 0.327971 |
Target: 5'- -cCGUCAGGGUCUUGUCcuGCUGGCGc -3' miRNA: 3'- cuGUAGUCCUAGAGCAGc-UGGCCGUc -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 41448 | 0.73 | 0.319994 |
Target: 5'- cGACcacgCAGGcgCUCGUCGACCGcGCc- -3' miRNA: 3'- -CUGua--GUCCuaGAGCAGCUGGC-CGuc -5' |
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18549 | 5' | -54.7 | NC_004682.1 | + | 30185 | 0.85 | 0.054503 |
Target: 5'- cGACGcCAGGAUCUCGUCgugGAUCGGCAGg -3' miRNA: 3'- -CUGUaGUCCUAGAGCAG---CUGGCCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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