Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18551 | 5' | -53.2 | NC_004682.1 | + | 45827 | 0.66 | 0.763601 |
Target: 5'- -aGCGACcGCGUCGgccaauugcucgccgUAGUaGGCGAGu -3' miRNA: 3'- uaCGCUGuUGCAGC---------------AUCGaCCGCUUg -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 25670 | 0.67 | 0.693812 |
Target: 5'- aGUGCGGCGAUGaCGuUGGCgaccuguucGGUGGACa -3' miRNA: 3'- -UACGCUGUUGCaGC-AUCGa--------CCGCUUG- -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 45780 | 0.67 | 0.693812 |
Target: 5'- gGUGCGGCGucgGCGUacgCGUAGCccuucGGCGGGa -3' miRNA: 3'- -UACGCUGU---UGCA---GCAUCGa----CCGCUUg -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 34728 | 0.68 | 0.633896 |
Target: 5'- gGUGCGcCcgguagagccccguGAUGUCGUAGCUGccguugccgugcGCGAACg -3' miRNA: 3'- -UACGCuG--------------UUGCAGCAUCGAC------------CGCUUG- -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 11799 | 0.68 | 0.648662 |
Target: 5'- gGUGcCGucuCGGCGUCGUGGaCcGGCGAGg -3' miRNA: 3'- -UAC-GCu--GUUGCAGCAUC-GaCCGCUUg -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 39405 | 0.68 | 0.660002 |
Target: 5'- -gGCGGUGAUGcCGUAGgUGGCGAAg -3' miRNA: 3'- uaCGCUGUUGCaGCAUCgACCGCUUg -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 4563 | 0.68 | 0.660002 |
Target: 5'- -gGCcGCAACGccgcguucgcUCGggcGCUGGCGAGCu -3' miRNA: 3'- uaCGcUGUUGC----------AGCau-CGACCGCUUG- -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 32754 | 0.69 | 0.580657 |
Target: 5'- -gGUGACGAguuCGUCGUAcgccucggcGCUGGCGuGCu -3' miRNA: 3'- uaCGCUGUU---GCAGCAU---------CGACCGCuUG- -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 25189 | 0.69 | 0.591934 |
Target: 5'- -cGCacucaACAGCGUCcaAGCUGGCGAGg -3' miRNA: 3'- uaCGc----UGUUGCAGcaUCGACCGCUUg -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 28083 | 0.69 | 0.591934 |
Target: 5'- -gGCGACGaagACGcucgggCGUGGCUgcucgcggcGGCGAGCg -3' miRNA: 3'- uaCGCUGU---UGCa-----GCAUCGA---------CCGCUUG- -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 15050 | 0.7 | 0.521853 |
Target: 5'- gGUGCGGC-GCGUggucgacgggaaccUGgcGCUGGUGGACg -3' miRNA: 3'- -UACGCUGuUGCA--------------GCauCGACCGCUUG- -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 40589 | 0.72 | 0.401943 |
Target: 5'- -gGCGAagaacuuggccCAACGcaugaCGUAGUUGGCGAGCg -3' miRNA: 3'- uaCGCU-----------GUUGCa----GCAUCGACCGCUUG- -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 17276 | 0.74 | 0.347608 |
Target: 5'- -gGCGACAACGgCGcucuGCUgGGCGAGCu -3' miRNA: 3'- uaCGCUGUUGCaGCau--CGA-CCGCUUG- -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 20573 | 0.79 | 0.164621 |
Target: 5'- -gGCGACAugGUCcuucggggcgugGUcGCUGGCGAGCg -3' miRNA: 3'- uaCGCUGUugCAG------------CAuCGACCGCUUG- -5' |
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18551 | 5' | -53.2 | NC_004682.1 | + | 42509 | 1.09 | 0.001366 |
Target: 5'- aAUGCGACAACGUCGUAGCUGGCGAACa -3' miRNA: 3'- -UACGCUGUUGCAGCAUCGACCGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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