Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18554 | 3' | -53 | NC_004682.1 | + | 26270 | 0.66 | 0.838273 |
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Target: 5'- cGCGUUCGCGggGa--CGuuGUUgCCGCc -3' miRNA: 3'- -CGCAGGUGCuuCaagGCuuCGA-GGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 33246 | 0.66 | 0.838273 |
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Target: 5'- cGUGUCCGCGAgcguGGUgaacgCUGGcgugAGCgCCGUg -3' miRNA: 3'- -CGCAGGUGCU----UCAa----GGCU----UCGaGGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 22240 | 0.66 | 0.84706 |
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Target: 5'- --cUCUACGggGggaggCCGGAucGCUCCGg -3' miRNA: 3'- cgcAGGUGCuuCaa---GGCUU--CGAGGCg -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 7499 | 0.66 | 0.84706 |
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Target: 5'- aGUGgccCCGCGAgagGGUcgCggaGAAGUUCCGCg -3' miRNA: 3'- -CGCa--GGUGCU---UCAa-Gg--CUUCGAGGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 49338 | 0.66 | 0.84706 |
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Target: 5'- -gGUCUucgGCGAAG-UCUGAAGCUCgaaGUa -3' miRNA: 3'- cgCAGG---UGCUUCaAGGCUUCGAGg--CG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 10870 | 0.66 | 0.84706 |
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Target: 5'- uCGUUCGCcGGGUUCCGGuucguccgugcGGCUUaCGCg -3' miRNA: 3'- cGCAGGUGcUUCAAGGCU-----------UCGAG-GCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 25628 | 0.66 | 0.854772 |
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Target: 5'- -gGUCCcguCGAAGUagcgcugUCCGGGcGCUCCa- -3' miRNA: 3'- cgCAGGu--GCUUCA-------AGGCUU-CGAGGcg -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 9409 | 0.66 | 0.855617 |
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Target: 5'- aCGgcaCgACGGAGaucaccCCGGAGCUUCGCu -3' miRNA: 3'- cGCa--GgUGCUUCaa----GGCUUCGAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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