Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18554 | 3' | -53 | NC_004682.1 | + | 5905 | 0.67 | 0.771147 |
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Target: 5'- gGCGUCCAccCGAAGUcgUgGgcGUUCCaGCa -3' miRNA: 3'- -CGCAGGU--GCUUCAa-GgCuuCGAGG-CG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 5138 | 0.66 | 0.829266 |
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Target: 5'- cGCGUgCUGCGGGcaucUCCGAGGCcaaggcCCGCg -3' miRNA: 3'- -CGCA-GGUGCUUca--AGGCUUCGa-----GGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 4731 | 0.72 | 0.52215 |
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Target: 5'- cGCGgCCACGAAGUggaucgaguaccacgCCGAAGCcgacgaCUGCg -3' miRNA: 3'- -CGCaGGUGCUUCAa--------------GGCUUCGa-----GGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 3561 | 0.7 | 0.597298 |
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Target: 5'- gGCGcuggCCGaGGAGaUCCGAGGCUugccCCGCg -3' miRNA: 3'- -CGCa---GGUgCUUCaAGGCUUCGA----GGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 3393 | 0.67 | 0.801016 |
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Target: 5'- uCGUaCACGccGacaCCGAGGCUCCGUu -3' miRNA: 3'- cGCAgGUGCuuCaa-GGCUUCGAGGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 2110 | 0.69 | 0.663871 |
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Target: 5'- -gGUCCGCGAGGguucaCCGGAGaagCCGg -3' miRNA: 3'- cgCAGGUGCUUCaa---GGCUUCga-GGCg -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 2065 | 0.69 | 0.663871 |
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Target: 5'- -gGUCCGC-AGGgUCCGAccGGC-CCGCa -3' miRNA: 3'- cgCAGGUGcUUCaAGGCU--UCGaGGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 1929 | 0.68 | 0.729332 |
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Target: 5'- gGCGUUCgucaaggacuGCGAcaagcgcaaGGcUCCGAAGCuguUCCGCa -3' miRNA: 3'- -CGCAGG----------UGCU---------UCaAGGCUUCG---AGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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