Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18554 | 3' | -53 | NC_004682.1 | + | 15286 | 0.66 | 0.810627 |
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Target: 5'- uCGuUCCGCGAAGaggCCGAccgcgAGUUCgCGCc -3' miRNA: 3'- cGC-AGGUGCUUCaa-GGCU-----UCGAG-GCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 41211 | 0.67 | 0.791225 |
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Target: 5'- cGCGgaUCUGCauGGUgucUCCGAugaacucaAGCUCCGCg -3' miRNA: 3'- -CGC--AGGUGcuUCA---AGGCU--------UCGAGGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 5138 | 0.66 | 0.829266 |
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Target: 5'- cGCGUgCUGCGGGcaucUCCGAGGCcaaggcCCGCg -3' miRNA: 3'- -CGCA-GGUGCUUca--AGGCUUCGa-----GGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 42244 | 0.67 | 0.785268 |
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Target: 5'- aGCGUCuuCAUGAucacGGggCCGggGUagccagcgcggugcaUCCGCa -3' miRNA: 3'- -CGCAG--GUGCU----UCaaGGCuuCG---------------AGGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 40002 | 0.67 | 0.781264 |
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Target: 5'- aGCGUCUgcGCGAGG--CCGAGGCccUCgGUg -3' miRNA: 3'- -CGCAGG--UGCUUCaaGGCUUCG--AGgCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 29913 | 0.67 | 0.771147 |
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Target: 5'- cGCGccgaUCCgGCGcAGUUCCuGGAuaccugcuGCUCCGCg -3' miRNA: 3'- -CGC----AGG-UGCuUCAAGG-CUU--------CGAGGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 22831 | 0.67 | 0.771147 |
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Target: 5'- aGCGgcCCGgaaGAAGgucCCGGAGC-CCGCg -3' miRNA: 3'- -CGCa-GGUg--CUUCaa-GGCUUCGaGGCG- -5' |
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| 18554 | 3' | -53 | NC_004682.1 | + | 23218 | 0.74 | 0.400949 |
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Target: 5'- cCGUCCGugaGggGUUCCGcGAGaUCCGCa -3' miRNA: 3'- cGCAGGUg--CuuCAAGGC-UUCgAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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