Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18556 | 3' | -54.2 | NC_004682.1 | + | 41073 | 0.68 | 0.619471 |
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Target: 5'- cACCAgUCGGcucuCCGAGGaC-CGGAUGGCc -3' miRNA: 3'- -UGGUgAGCUu---GGCUUC-GaGCCUGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 41984 | 0.74 | 0.299968 |
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Target: 5'- uACgGCgUCGGGCCGgcGCUCGGACucauAGUa -3' miRNA: 3'- -UGgUG-AGCUUGGCuuCGAGCCUG----UCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 42525 | 0.7 | 0.51048 |
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Target: 5'- --uGCUCGAuguacCCGAcguGGCUCGG-CGGCg -3' miRNA: 3'- uggUGAGCUu----GGCU---UCGAGCCuGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 43600 | 0.67 | 0.685932 |
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Target: 5'- cACCACagcgUGAACCGAgccuguuccAGCgUCGGcaugaACGGCg -3' miRNA: 3'- -UGGUGa---GCUUGGCU---------UCG-AGCC-----UGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 45548 | 0.67 | 0.718601 |
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Target: 5'- cCCGCcUGcGCCGggGC-CGGcgugaGCAGCa -3' miRNA: 3'- uGGUGaGCuUGGCuuCGaGCC-----UGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 47077 | 0.69 | 0.608374 |
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Target: 5'- aGCCGCcCGGAUagGAGGUgguugccCGGACGGCg -3' miRNA: 3'- -UGGUGaGCUUGg-CUUCGa------GCCUGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 47436 | 0.72 | 0.391747 |
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Target: 5'- cGCC-CUCGAACgGAGcCUCGGuguCGGCg -3' miRNA: 3'- -UGGuGAGCUUGgCUUcGAGCCu--GUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 49249 | 0.66 | 0.739964 |
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Target: 5'- uGCCACUUGGugCGAuccgggauGGCgUCGGcguCGGUc -3' miRNA: 3'- -UGGUGAGCUugGCU--------UCG-AGCCu--GUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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