Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18556 | 3' | -54.2 | NC_004682.1 | + | 15238 | 0.66 | 0.778245 |
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Target: 5'- aGCC-CUCGGACCacuucGAguucacguucaugcGGCUCauggaGGGCGGCg -3' miRNA: 3'- -UGGuGAGCUUGG-----CU--------------UCGAG-----CCUGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 25568 | 0.66 | 0.739964 |
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Target: 5'- cGCCACUgGGccacGCCGuGGGCcuugacgCGGugGGCu -3' miRNA: 3'- -UGGUGAgCU----UGGC-UUCGa------GCCugUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 5417 | 0.66 | 0.739964 |
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Target: 5'- gACCACucaUCGAGCgGAGGUgaggCGcGAaaCAGCa -3' miRNA: 3'- -UGGUG---AGCUUGgCUUCGa---GC-CU--GUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 49249 | 0.66 | 0.739964 |
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Target: 5'- uGCCACUUGGugCGAuccgggauGGCgUCGGcguCGGUc -3' miRNA: 3'- -UGGUGAGCUugGCU--------UCG-AGCCu--GUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 36834 | 0.66 | 0.729332 |
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Target: 5'- uGCCGC-CgGGGCCGAAcacacCGGACGGCu -3' miRNA: 3'- -UGGUGaG-CUUGGCUUcga--GCCUGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 43600 | 0.67 | 0.685932 |
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Target: 5'- cACCACagcgUGAACCGAgccuguuccAGCgUCGGcaugaACGGCg -3' miRNA: 3'- -UGGUGa---GCUUGGCU---------UCG-AGCC-----UGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 38317 | 0.67 | 0.674922 |
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Target: 5'- gACCAUgaacgCGAACUc-AGCUucgcaaCGGACAGCg -3' miRNA: 3'- -UGGUGa----GCUUGGcuUCGA------GCCUGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 40407 | 1.12 | 0.00073 |
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Target: 5'- aACCACUCGAACCGAAGCUCGGACAGCa -3' miRNA: 3'- -UGGUGAGCUUGGCUUCGAGCCUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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