Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18556 | 3' | -54.2 | NC_004682.1 | + | 38019 | 0.67 | 0.69689 |
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Target: 5'- cGCCucCUCGGuGCCGAGGUgccucagCGccGACAGCg -3' miRNA: 3'- -UGGu-GAGCU-UGGCUUCGa------GC--CUGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 9436 | 0.67 | 0.674922 |
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Target: 5'- uUCGCUCGGucACCGAcgcAGCUgccCGGAC-GCu -3' miRNA: 3'- uGGUGAGCU--UGGCU---UCGA---GCCUGuCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 6857 | 0.67 | 0.674922 |
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Target: 5'- gGCCcgaACUcCGuugugucccAACUGAucgAGCUUGGACAGCg -3' miRNA: 3'- -UGG---UGA-GC---------UUGGCU---UCGAGCCUGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 21316 | 0.68 | 0.640578 |
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Target: 5'- gACaACUCGGGCUGggGCagugagaccgccuUCGG-CGGCu -3' miRNA: 3'- -UGgUGAGCUUGGCuuCG-------------AGCCuGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 11509 | 0.68 | 0.630579 |
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Target: 5'- cCCACUCa-----GGGCUCGGGCAGCc -3' miRNA: 3'- uGGUGAGcuuggcUUCGAGCCUGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 47077 | 0.69 | 0.608374 |
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Target: 5'- aGCCGCcCGGAUagGAGGUgguugccCGGACGGCg -3' miRNA: 3'- -UGGUGaGCUUGg-CUUCGa------GCCUGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 4949 | 0.69 | 0.608374 |
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Target: 5'- gGCUACgaCGAcacccCCGguGCUCGGGCGGUc -3' miRNA: 3'- -UGGUGa-GCUu----GGCuuCGAGCCUGUCG- -5' |
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| 18556 | 3' | -54.2 | NC_004682.1 | + | 18338 | 0.85 | 0.059992 |
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Target: 5'- gGCCcguuCUCGGGCCGAGGCUgGGugAGCu -3' miRNA: 3'- -UGGu---GAGCUUGGCUUCGAgCCugUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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