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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18556 | 5' | -62.2 | NC_004682.1 | + | 50540 | 0.66 | 0.305319 |
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Target: 5'- gUGGGGUaCCcGCUaCCGGGgguacugGGUacuGGGGg -3' miRNA: 3'- aACCCCA-GGuCGA-GGCCCa------CCA---CCUC- -5' |
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| 18556 | 5' | -62.2 | NC_004682.1 | + | 20756 | 0.67 | 0.283156 |
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Target: 5'- -cGGuGGUCCGGgUucauggaucacuaCCGGGUGGUccgcgaGGAGa -3' miRNA: 3'- aaCC-CCAGGUCgA-------------GGCCCACCA------CCUC- -5' |
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| 18556 | 5' | -62.2 | NC_004682.1 | + | 43785 | 0.67 | 0.276965 |
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Target: 5'- -cGGGG-CaAGCUCCGGGUGaUGGu- -3' miRNA: 3'- aaCCCCaGgUCGAGGCCCACcACCuc -5' |
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| 18556 | 5' | -62.2 | NC_004682.1 | + | 15852 | 0.67 | 0.263595 |
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Target: 5'- cUGGaGGUCgaugacgAGUUCUGGGUGGccgUGGAGg -3' miRNA: 3'- aACC-CCAGg------UCGAGGCCCACC---ACCUC- -5' |
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| 18556 | 5' | -62.2 | NC_004682.1 | + | 40442 | 1.05 | 0.000329 |
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Target: 5'- gUUGGGGUCCAGCUCCGGGUGGUGGAGu -3' miRNA: 3'- -AACCCCAGGUCGAGGCCCACCACCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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