Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18557 | 3' | -56.3 | NC_004682.1 | + | 50757 | 0.66 | 0.623357 |
Target: 5'- -cGCCCGCga--GGGCggcUCUGAGCCu -3' miRNA: 3'- cuCGGGCGaagaCCCGa--AGGUUUGGc -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 45849 | 0.66 | 0.612271 |
Target: 5'- uAGCCCuGCggCUGGaagccGCUgucggcgaUCCAGACCGc -3' miRNA: 3'- cUCGGG-CGaaGACC-----CGA--------AGGUUUGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 4899 | 0.66 | 0.59016 |
Target: 5'- uGAGCCCGCgcuaCUcGGGCggcaCCAcguACCa -3' miRNA: 3'- -CUCGGGCGaa--GA-CCCGaa--GGUu--UGGc -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 18142 | 0.66 | 0.59016 |
Target: 5'- aGGCCCGCgaagCggccaGGGCggUCCu-GCCGa -3' miRNA: 3'- cUCGGGCGaa--Ga----CCCGa-AGGuuUGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 39607 | 0.67 | 0.557285 |
Target: 5'- cAGCCCGCguagcggUCUuGGCcUCCucGACCGc -3' miRNA: 3'- cUCGGGCGa------AGAcCCGaAGGu-UUGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 23006 | 0.67 | 0.557285 |
Target: 5'- gGGGCCguccuuuucgCGUUUCUggGGGUUUCC-GACCGg -3' miRNA: 3'- -CUCGG----------GCGAAGA--CCCGAAGGuUUGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 27070 | 0.67 | 0.535657 |
Target: 5'- -cGCCCuCcagUUCUGGGCUuagCCAGggGCCGa -3' miRNA: 3'- cuCGGGcG---AAGACCCGAa--GGUU--UGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 38011 | 0.67 | 0.524953 |
Target: 5'- gGGGCCU-CUgc-GGGCUUCUGAGCCGc -3' miRNA: 3'- -CUCGGGcGAagaCCCGAAGGUUUGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 46477 | 0.68 | 0.514331 |
Target: 5'- -cGCUCGCUcacggUCUGGGCgaagccugcgaUCCAGGCgCGg -3' miRNA: 3'- cuCGGGCGA-----AGACCCGa----------AGGUUUG-GC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 3906 | 0.68 | 0.493359 |
Target: 5'- cAGCCCGCUUCggcGGGCcUCauuGAUCu -3' miRNA: 3'- cUCGGGCGAAGa--CCCGaAGgu-UUGGc -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 13612 | 0.68 | 0.472787 |
Target: 5'- cGAGCaCgGCgUUCUGGGCgcucagUCCgAGAUCGa -3' miRNA: 3'- -CUCG-GgCG-AAGACCCGa-----AGG-UUUGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 2460 | 0.68 | 0.462665 |
Target: 5'- gGGGCgaUGCUcCUGGGCgaCCAGAUCGa -3' miRNA: 3'- -CUCGg-GCGAaGACCCGaaGGUUUGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 30740 | 0.69 | 0.433004 |
Target: 5'- cAGCCaguCGCcgCUGGGCagcgUCUGAGCCGg -3' miRNA: 3'- cUCGG---GCGaaGACCCGa---AGGUUUGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 10087 | 0.7 | 0.359667 |
Target: 5'- -uGCCCGCcagUC-GGGCUggccggUCCAGAUCGa -3' miRNA: 3'- cuCGGGCGa--AGaCCCGA------AGGUUUGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 19093 | 0.71 | 0.351131 |
Target: 5'- -cGUCCggGCUcaUCUGGGCUUCCucgGACUGg -3' miRNA: 3'- cuCGGG--CGA--AGACCCGAAGGu--UUGGC- -5' |
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18557 | 3' | -56.3 | NC_004682.1 | + | 39993 | 1.09 | 0.000585 |
Target: 5'- aGAGCCCGCUUCUGGGCUUCCAAACCGg -3' miRNA: 3'- -CUCGGGCGAAGACCCGAAGGUUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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