Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18557 | 5' | -53.7 | NC_004682.1 | + | 33907 | 0.66 | 0.764292 |
Target: 5'- gCCGCCUUGAucccguccaugccgaGGccacagcgccgacACCGGCACCGa--- -3' miRNA: 3'- aGGUGGAACU---------------UC-------------UGGCCGUGGUgaaa -5' |
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18557 | 5' | -53.7 | NC_004682.1 | + | 42134 | 0.66 | 0.761131 |
Target: 5'- aCCGCCcuccGGGugCGGUugCGCUUa -3' miRNA: 3'- aGGUGGaac-UUCugGCCGugGUGAAa -5' |
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18557 | 5' | -53.7 | NC_004682.1 | + | 11484 | 0.66 | 0.761131 |
Target: 5'- uUCCAa---GGAGGCCGGUGCUACg-- -3' miRNA: 3'- -AGGUggaaCUUCUGGCCGUGGUGaaa -5' |
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18557 | 5' | -53.7 | NC_004682.1 | + | 47547 | 0.66 | 0.739755 |
Target: 5'- gCCGCCgcugccgaaggUGAAcguauGGCUGGCACCGCc-- -3' miRNA: 3'- aGGUGGa----------ACUU-----CUGGCCGUGGUGaaa -5' |
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18557 | 5' | -53.7 | NC_004682.1 | + | 28752 | 0.66 | 0.739755 |
Target: 5'- gCCgAUCUUGGAGACCcGCuCCACg-- -3' miRNA: 3'- aGG-UGGAACUUCUGGcCGuGGUGaaa -5' |
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18557 | 5' | -53.7 | NC_004682.1 | + | 17908 | 0.66 | 0.728887 |
Target: 5'- gUCCugACCgagcUGGAGcgucACCGGCACCugUc- -3' miRNA: 3'- -AGG--UGGa---ACUUC----UGGCCGUGGugAaa -5' |
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18557 | 5' | -53.7 | NC_004682.1 | + | 19012 | 0.66 | 0.717916 |
Target: 5'- -gCACCggcaUGAGGucGCgGGCACCACg-- -3' miRNA: 3'- agGUGGa---ACUUC--UGgCCGUGGUGaaa -5' |
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18557 | 5' | -53.7 | NC_004682.1 | + | 31213 | 0.67 | 0.684512 |
Target: 5'- aUCCugCgaGAccGCCGGCACCAg--- -3' miRNA: 3'- -AGGugGaaCUucUGGCCGUGGUgaaa -5' |
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18557 | 5' | -53.7 | NC_004682.1 | + | 137 | 0.67 | 0.673254 |
Target: 5'- aCCGCCggcacGGGCCGGCacaggGCCACa-- -3' miRNA: 3'- aGGUGGaacu-UCUGGCCG-----UGGUGaaa -5' |
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18557 | 5' | -53.7 | NC_004682.1 | + | 17673 | 0.7 | 0.494728 |
Target: 5'- aUCCACCguccucGAagaucccuacuGGGCCGGCACCgGCUa- -3' miRNA: 3'- -AGGUGGaa----CU-----------UCUGGCCGUGG-UGAaa -5' |
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18557 | 5' | -53.7 | NC_004682.1 | + | 40028 | 1.07 | 0.00161 |
Target: 5'- gUCCACCUUGAAGACCGGCACCACUUUg -3' miRNA: 3'- -AGGUGGAACUUCUGGCCGUGGUGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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