Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18560 | 5' | -50.6 | NC_004682.1 | + | 40251 | 0.66 | 0.931062 |
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Target: 5'- aUGCGggGAgCGgGGUcGUCUUUgACCGc -3' miRNA: 3'- -ACGCuuCUaGCaCCAcUAGAAG-UGGC- -5' |
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| 18560 | 5' | -50.6 | NC_004682.1 | + | 32029 | 0.66 | 0.925202 |
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Target: 5'- cGCGGAGAccUgGUcGGUGuaCUUCGCCu -3' miRNA: 3'- aCGCUUCU--AgCA-CCACuaGAAGUGGc -5' |
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| 18560 | 5' | -50.6 | NC_004682.1 | + | 29302 | 0.66 | 0.912594 |
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Target: 5'- aGCGAugagcuuccGGGUCuccUGGUGGUCggUGCCGa -3' miRNA: 3'- aCGCU---------UCUAGc--ACCACUAGaaGUGGC- -5' |
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| 18560 | 5' | -50.6 | NC_004682.1 | + | 41423 | 0.67 | 0.876003 |
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Target: 5'- aGCGAAGcUCcggGGUGAUCUccgUCguGCCGu -3' miRNA: 3'- aCGCUUCuAGca-CCACUAGA---AG--UGGC- -5' |
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| 18560 | 5' | -50.6 | NC_004682.1 | + | 1958 | 0.68 | 0.850767 |
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Target: 5'- gGCGAgaucuGGAUCGcUGGcGGUCUgcugUACCGc -3' miRNA: 3'- aCGCU-----UCUAGC-ACCaCUAGAa---GUGGC- -5' |
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| 18560 | 5' | -50.6 | NC_004682.1 | + | 29629 | 0.69 | 0.773434 |
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Target: 5'- cGCGcAGAUCcucgaacgGGUGcggGUCUUCGCCGu -3' miRNA: 3'- aCGCuUCUAGca------CCAC---UAGAAGUGGC- -5' |
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| 18560 | 5' | -50.6 | NC_004682.1 | + | 35142 | 0.73 | 0.572646 |
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Target: 5'- cGCGAuGG-CGUGGUcGAUCUccaUCGCCGc -3' miRNA: 3'- aCGCUuCUaGCACCA-CUAGA---AGUGGC- -5' |
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| 18560 | 5' | -50.6 | NC_004682.1 | + | 38957 | 1.11 | 0.002288 |
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Target: 5'- cUGCGAAGAUCGUGGUGAUCUUCACCGg -3' miRNA: 3'- -ACGCUUCUAGCACCACUAGAAGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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