Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18561 | 3' | -60.6 | NC_004682.1 | + | 30643 | 0.69 | 0.275635 |
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Target: 5'- gGUCGCCC--GACaGCgCGGCCaggACGCGGa -3' miRNA: 3'- -CAGCGGGuaCUG-CG-GCUGG---UGCGCCa -5' |
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| 18561 | 3' | -60.6 | NC_004682.1 | + | 37232 | 0.69 | 0.254001 |
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Target: 5'- -gCGCCCAgaACGCCGugCucggcgucccaggaGCGCGGg -3' miRNA: 3'- caGCGGGUacUGCGGCugG--------------UGCGCCa -5' |
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| 18561 | 3' | -60.6 | NC_004682.1 | + | 26174 | 0.7 | 0.247726 |
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Target: 5'- cGUCGCCCcgcaucgagaugccGUugcaguugagcGGCGuCCGACCGCaGCGGUa -3' miRNA: 3'- -CAGCGGG--------------UA-----------CUGC-GGCUGGUG-CGCCA- -5' |
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| 18561 | 3' | -60.6 | NC_004682.1 | + | 32674 | 0.7 | 0.231424 |
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Target: 5'- aUCGCCCAgaGCGCUcuuGACCAUGcCGGg -3' miRNA: 3'- cAGCGGGUacUGCGG---CUGGUGC-GCCa -5' |
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| 18561 | 3' | -60.6 | NC_004682.1 | + | 49308 | 0.7 | 0.231424 |
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Target: 5'- cGUCGCCgCGugcguUGACGCUGAUCACuuuGUGGUc -3' miRNA: 3'- -CAGCGG-GU-----ACUGCGGCUGGUG---CGCCA- -5' |
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| 18561 | 3' | -60.6 | NC_004682.1 | + | 46465 | 0.7 | 0.219939 |
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Target: 5'- -gCGCCCGUGGCGgCGGCUucggugACgGCGGUc -3' miRNA: 3'- caGCGGGUACUGCgGCUGG------UG-CGCCA- -5' |
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| 18561 | 3' | -60.6 | NC_004682.1 | + | 41325 | 0.71 | 0.197913 |
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Target: 5'- cUCGCCCuUGACGCCGaacagcaGCCGCuGgGGg -3' miRNA: 3'- cAGCGGGuACUGCGGC-------UGGUG-CgCCa -5' |
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| 18561 | 3' | -60.6 | NC_004682.1 | + | 38487 | 1.06 | 0.000473 |
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Target: 5'- aGUCGCCCAUGACGCCGACCACGCGGUu -3' miRNA: 3'- -CAGCGGGUACUGCGGCUGGUGCGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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